ERK2 | HUABIO EM1901-54 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ERK2

catalog :

EM1901-54

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A10D2

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (EM1901-54) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: Human brain tissue lysate (40 µg/Lane) Lane 12: Mouse brain tissue lysate (40 µg/Lane) Lane 13: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: Lane 1-11: 1 minute 30 seconds; Lane 12-13: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1901-54

Target name :

ERK2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human ERK2 aa 200-360.

Uniprot id :

P28482>SwissProt: P28482 Human;SwissProt: P63085 Mouse;SwissProt: P63086 Rat

Host :

Mouse

Clone number :

A10D2

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human thyroid tissue, human skin tissue, human breast carcinoma tissue, human pancreas tissue, mouse testis tissue, mouse colon tissue, mouse ovary tissue, K562.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Nucleus, spindle, centrosome, cytoplasm, caveola.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-54_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1901-54_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1901-54_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1901-54_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/EM1901-54_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/EM1901-54_9.jpg

Pic legend9 :

Flow cytometric analysis of ERK2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-54, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/EM1901-54_10.jpg

Pic legend10 :

Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (EM1901-54) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate (10 µg/Lane) Lane 2: A549-si ERK2 cell lysate (10 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-54) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage