product summary
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company name :
HUABIO
product type :
antibody
product name :
DFNA5 / GSDME
catalog :
EM1901-49
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A1H2
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of DFNA5 / GSDME on different lysates with Mouse anti-DFNA5 / GSDME antibody (EM1901-49) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: SW480 cell lysate (negative)
Lane 3: SGC-7901 cell lysate
Lane 4: MCF7 cell lysate (negative)
Lane 5: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-49) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
EM1901-49
Target name :
DFNA5 / GSDME
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human DFNA5 aa 34-214 / 496.
Uniprot id :
O60443>SwissProt: O60443 Human
Host :
Mouse
Clone number :
A1H2
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
HeLa cell lysate, SGC-7901 cell lysate, SH-SY5Y cell lysate.
Molecular wt :
Predicted band size: 55 kDa
Subcellular location :
Cell membrane, cytosol.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
Advanced Validation :
Relative expression (RE)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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