Dynamin 1 | HUABIO EM1901-44 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Dynamin 1

catalog :

EM1901-44

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A1A12

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of MG-63 cells labeling Dynamin 1 with Mouse anti-Dynamin 1 antibody (EM1901-44) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Dynamin 1 antibody (EM1901-44) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 2 :

ICC staining of Dynamin 1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-44, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Flow cytometric analysis of A549 cells labeling Dynamin 1. Cells were fixed and permeabilized.Then stained with the primary antibody (EM1901-44, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

EM1901-44

Target name :

Dynamin 1

Species reactivity :

Human

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Dynamin 1 aa 555-770 / 864.

Uniprot id :

Q05193>SwissProt: Q05193 Human

Host :

Mouse

Clone number :

A1A12

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

MG-63, SiHa, A549, rat brain tissue lysate, mouse brain tissue lysate.

Molecular wt :

Predicted band size: 97 kDa

Subcellular location :

Cytoskeleton, cytoplasm.

Concentration :

2 mg/mL.

Recommended dilutions :

WB:1:500-1:2,000 ;IF-Cell:1:50-1:200 ;FC:1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-44_4.jpg

Pic legend4 :

Western blot analysis of Dynamin 1 on different lysates with Mouse anti-Dynamin 1 antibody (EM1901-44) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-44) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage