CD10 | HUABIO EM1901-24 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD10

catalog :

EM1901-24

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A1G3

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD10 on different lysates with Mouse anti-CD10 antibody (EM1901-24) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane) Lane 2: Ramos cell lysate (20 µg/Lane) Lane 3: LNCaP cell lysate (20 µg/Lane) Lane 4: HT-29 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (40 µg/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Mouse anti-CD10 antibody (EM1901-24) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-24) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD10 antibody (EM1901-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1901-24

Target name :

CD10

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,FC,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human CD10 aa 200-300.

Uniprot id :

P08473>SwissProt: P08473 Human;SwissProt: Q61391 Mouse

Host :

Mouse

Clone number :

A1G3

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Daudi cell lysate, Ramos cell lysate, LNCaP cell lysate, mouse kidney tissue lysate, human renal clear cell carcinoma tissue, human kidney tissue, human prostate tissue, 293.

Molecular wt :

Predicted band size: 86 kDa

Subcellular location :

Cell membrane

Concentration :

2 mg/mL

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:200-1:1,000 ;FC: 1:50-1:100 ;IF-Tissue: 1:50

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-24_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1901-24_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling CD10 with Mouse anti-CD10 antibody (EM1901-24) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1901-24, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1901-24_6.jpg

Pic legend6 :

Flow cytometric analysis of CD10 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-24, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage