Cathepsin D | HUABIO EM1901-17 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cathepsin D

catalog :

EM1901-17

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13F4

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cathepsin D on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: MCF-7 cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Cathepsin D antibody (EM1901-17) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-Cathepsin D antibody (EM1901-17) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1901-17

Target name :

Cathepsin D

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Cathepsin D aa 10-412.

Uniprot id :

P07339>SwissProt: P07339 Human

Host :

Mouse

Clone number :

13F4

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

SK-Br-3 cell lysate, MCF-7 cell lysate, human breast cancer tissue, human liver cancer tissue, human liver tissue.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Lysosome, extracellular space, melanosome.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:1,000-1:5,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-17_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cathepsin D antibody (EM1901-17) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-17) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage