NQO1 | HUABIO EM1801-21 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NQO1

catalog :

EM1801-21

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13A1

reactivity :

human, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (EM1801-21) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: SiHa cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: HeLa cell lysate Lane 6: Saos-2 cell lysate (low expression) Lysates/proteins at 30 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (EM1801-21) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si NQO1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

EM1801-21

Target name :

NQO1

Species reactivity :

Human,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human NQO1 aa 1-274 / 274.

Uniprot id :

P15559>SwissProt: P15559 Human;SwissProt: P05982 Rat

Host :

Mouse

Clone number :

13A1

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A549 cell lysate, SiHa cell lysate, HepG2 cell lysate, MCF7 cell lysate, HeLa cell lysate.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Cytoplasm

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000

Advanced Validation :

Relative expression (RE),Knockdown (KD)

more info or order :

HUABIO product webpage