HLA Class 1 ABC | HUABIO EM1801-09 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HLA Class 1 ABC

catalog :

EM1801-09

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

11C3

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HLA Class 1 ABC on different lysates with Mouse anti-HLA Class 1 ABC antibody (EM1801-09) at 1/1,000 dilution. Lane 1: CAL-62 cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-09) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of HLA Class 1 ABC on different lysates with Mouse anti-HLA Class 1 ABC antibody (EM1801-09) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-HLA Class 1 ABC KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-09) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-HLA Class 1 ABC antibody (EM1801-09) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-09) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1801-09

Target name :

HLA Class 1 ABC

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human HLA Class 1 ABC aa 1-362 / 362.

Uniprot id :

P04439>SwissProt: P04439 Human;SwissProt: P01889 Human;SwissProt: P10321 Human

Host :

Mouse

Clone number :

11C3

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

CAL-62 cell lysate, HeLa cell lysate, HepG2 cell lysate, human liver tissue, 293T.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Cell Membrane, Endoplasmic reticulum membrane.

Concentration :

2 mg/mL.

Recommended dilutions :

WB:1:1,000-1:2000 ;IF-Cell:1:50-1:200 ;IHC-P: 1:5,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage