ALDH1A1 | HUABIO EM1801-07 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ALDH1A1

catalog :

EM1801-07

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8F1

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ALDH1A1 on different lysates with Mouse anti-ALDH1A1 antibody (EM1801-07) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Lane 6: Human lung tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-07) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ALDH1A1 on different lysates with Mouse anti-ALDH1A1 antibody (EM1801-07) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ALDH1A1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-07) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of A549 cells labeling ALDH1A1 with Mouse anti-ALDH1A1 antibody (EM1801-07) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH1A1 antibody (EM1801-07) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

EM1801-07

Target name :

ALDH1A1

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IHC-P,IF-Cell,FC,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ALDH1A1 aa 1-501 / 501.

Uniprot id :

P00352>SwissProt: P00352 Human;SwissProt: P51647 Rat

Host :

Mouse

Clone number :

8F1

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A549 cell lysate, HT-29 cell lysate, HepG2 cell lysate, K-562 cell lysate, human liver tissue lysate, human lung tissue lysate, A549, rat epididymis tissue, human liver tissue, human pancreas tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, mouse substantia nigra tissue.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Cell projection, Cytoplasm.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:10,000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IHC-Fr: 1:50

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1801-07_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-ALDH1A1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1801-07_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALDH1A1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1801-07_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ALDH1A1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1801-07_7.jpg

Pic legend7 :

Flow cytometric analysis of A549 cells labeling ALDH1A1. Cells were fixed and permeabilized. Then stained with the primary antibody (EM1801-07, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/EM1801-07_8.jpg

Pic legend8 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling ALDH1A1 with Mouse anti-ALDH1A1 antibody (EM1801-07). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (EM1801-07, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/EM1801-07_9.jpg

Pic legend9 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling ALDH1A1 with Mouse anti-ALDH1A1 antibody (EM1801-07). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (EM1801-07, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/EM1801-07_10.jpg

Pic legend10 :

Immunofluorescence analysis of frozen mouse substantia nigra tissue labeling ALDH1A1 with Mouse anti-ALDH1A1 antibody (EM1801-07). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (EM1801-07, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

more info or order :

HUABIO product webpage