product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
MSH2
catalog :
EM1801-04
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10G1
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (EM1801-04) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HEK-293 cell lysate (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: LNCAP cell lysate (negative) (15 µg/Lane)
Lane 5: Mouse testis tissue lysate (20 µg/Lane)
Lane 6: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-04) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
All lanes: Western blot analysis of MSH2 with anti-MSH2 antibody (EM1801-04) at 1/1,000 dilution.
Lane 1: Wild-type SCC7 whole cell lysate.
Lane 2: MSH2 knockout SCC7 whole cell lysate.
EM1801-04 was shown to specifically react with MSH2 in Wild-type SCC7 cells. No band was observed when MSH2 knockout sample was tested. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MSH2 antibody (EM1801-04, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MSH2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-04, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
EM1801-04
Target name :
MSH2
Species reactivity :
Human,Rat,Mouse
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within N-terminal human MSH2.
Uniprot id :
P43246>SwissProt: P43246 Human;SwissProt: P43247 Mouse;SwissProt: P54275 Rat
Host :
Mouse
Clone number :
10G1
Isotype :
IgG2b
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
HeLa cell lysate, HEK-293 cell lysate, A549 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, Wild-type SCC7 whole cell lysates, human breast carcinoma tissue, human placenta tissue, rat brain tissue, human kidney tissue.
Molecular wt :
Predicted band size: 105 kDa
Subcellular location :
Nucleus, Chromosome.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
;IHC-P: 1:100-1:1,000
Advanced Validation :
Relative expression (RE),Knockout (KO)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/EM1801-04_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MSH2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-04, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/EM1801-04_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MSH2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-04, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/EM1801-04_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MSH2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-04, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/EM1801-04_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin embedded human colon cancer tissue using anti-MSH2 antibody (1/200) performed on the Ventana® BenchMark ULTRA.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/EM1801-04_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin embedded human colon cancer tissue using anti-MSH2 antibody (1/200) performed on the Dako Omnis.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
related products
browse more products
questions and comments