IMP3 | HUABIO EM1801-01 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IMP3

catalog :

EM1801-01

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4-C2-5

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1801-01) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (40 µg/Lane) Lane 5: Mouse placenta tissue lysate (40 µg/Lane) Lane 6: Rat spleen tissue lysate (40 µg/Lane) Lane 7: Rat placenta tissue lysate (40 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: Lane 1-4: 20 seconds; Lane 5-6: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-01) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1801-01) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IMP3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-01) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Flow cytometric analysis of PANC-1 cells with IMP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

product information

SKU :

EM1801-01

Target name :

IMP3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human IMP3 aa 350-579.

Uniprot id :

O00425>SwissProt: O00425 Human;SwissProt: Q9CPN8 Mouse;Entrez Gene: 315697 Rat

Host :

Mouse

Clone number :

4-C2-5

Isotype :

IgG

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, MDA-MB-231 cell lysate, 293T cell lysate, Mouse spleen tissue lysate, Mouse placenta tissue lysate, Rat spleen tissue lysate, Rat placenta tissue lysate, PANC-1.

Molecular wt :

Predicted band size: 64 kDa

Subcellular location :

Nucleus.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage