product summary
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company name :
HUABIO
product type :
antibody
product name :
p53
catalog :
EM1701-92
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6B5
reactivity :
human
application :
western blot
more info or order :
image
image 1 :

Western blot analysis of p53 on 293T cell lysates with Mouse anti-p53 antibody (EM1701-92) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-92) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
product information
SKU :
EM1701-92
Target name :
p53
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within C-terminal human p53.
Uniprot id :
P04637>SwissProt: P04637 Human
Host :
Mouse
Clone number :
6B5
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
293T cell lysates.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
more info or order :
company information

HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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