PGP9.5 | HUABIO EM1701-86 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PGP9.5

catalog :

EM1701-86

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

B1-5-6

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PGP9.5 on different lysates with Mouse anti-PGP9.5 antibody (EM1701-86) at 1/2,000 dilution. Lane 1: A-172 cell lysate (20 µg/Lane) Lane 2: SHG-44 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: NCI-H1299 cell lysate (20 µg/Lane) Lane 6: A549 cell lysate (20 µg/Lane) Lane 7: 293T cell lysate (20 µg/Lane) Lane 8: LNCaP cell lysate (negative) (20 µg/Lane) Lane 9: K-562 cell lysate (negative) (20 µg/Lane) Lane 10: Neuro-2a cell lysate (20 µg/Lane) Lane 11: C6 cell lysate (20 µg/Lane) Lane 12: PC-12 cell lysate (20 µg/Lane) Lane 13: Mouse brain tissue lysate (20 µg/Lane) Lane 14: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-86) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PGP9.5 on different lysates with Mouse anti-PGP9.5 antibody (EM1701-86) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate (10 µg/Lane) Lane 2: A549-si PGP9.5 cell lysate (10 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-86) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 with Mouse anti-PGP9.5 antibody (EM1701-86) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PGP9.5 antibody (EM1701-86) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

EM1701-86

Target name :

PGP9.5

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PGP95 aa 174-223 / 223.

Uniprot id :

P09936>SwissProt: P09936 Human;SwissProt: Q9R0P9 Mouse;SwissProt: Q00981 Rat

Host :

Mouse

Clone number :

B1-5-6

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A-172 cell lysate, SHG-44 cell lysate, U-87 MG cell lysate, SH-SY5Y cell lysate, NCI-H1299 cell lysate, A549 cell lysate, 293T cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY-5Y, rat cerebellum tissue, human pancreas tissue, human kidney tissue, mouse brain tissue, Neuro-2a, PC-12.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Cytoplasm. Endoplasmic reticulum.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:50-1:200 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE),Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-86_4.jpg

Pic legend4 :

Immunocytochemistry analysis of PC-12 cells labeling PGP9.5 with Mouse anti-PGP9.5 antibody (EM1701-86) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PGP9.5 antibody (EM1701-86) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1701-86_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1701-86_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1701-86_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/EM1701-86_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/EM1701-86_9.jpg

Pic legend9 :

Flow cytometric analysis of Neuro-2a cells labeling PGP9.5. Cells were fixed and permeabilized. Then stained with the primary antibody (EM1701-86, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/EM1701-86_10.jpg

Pic legend10 :

Flow cytometric analysis of PC-12 cells labeling PGP9.5. Cells were fixed and permeabilized. Then stained with the primary antibody (EM1701-86, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage