product summary
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company name :
HUABIO
product type :
antibody
product name :
CDCP1
catalog :
EM1701-84
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A10F10
reactivity :
human, mouse
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of CDCP1 on different lysates with Mouse anti-CDCP1 antibody (EM1701-84) at 1/1,000 dilution.
Lane 1: SW480 cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: NIH/3T3 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 130 kDa
Exposure time: Lane 1-2: 24 seconds; Lane 3: 10 seconds;
ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of CDCP1 on different lysates with Mouse anti-CDCP1 antibody (EM1701-84) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: HCT 116 cell lysate treated with deglycosylation
Lysates/proteins at 14 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 93/130 kDa (Glycosylated)
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
EM1701-84
Target name :
CDCP1
Species reactivity :
Human,Mouse
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human CDCP1 aa 487-836 / 836.
Uniprot id :
Q9H5V8>SwissProt: Q9H5V8 Human;SwissProt: Q5U462 Mouse
Host :
Mouse
Clone number :
A10F10
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
SW480 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate.
Molecular wt :
Predicted band size: 93 kDa
Subcellular location :
Cell membrane. Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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