Bcl-2 | HUABIO EM1701-83 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Bcl-2

catalog :

EM1701-83

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9F3

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (EM1701-83) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-83) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Bcl-2 antibody (EM1701-83) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-83) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human b-cell lymphoma tissue with Mouse anti-Bcl-2 antibody (EM1701-83) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-83) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1701-83

Target name :

Bcl-2

Species reactivity :

Human

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human BCL2 aa 30-80.

Uniprot id :

P10415>SwissProt: P10415 Human

Host :

Mouse

Clone number :

9F3

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

THP-1 cell lysate, HL-60 cell lysate, human tonsil tissue, human b-cell lymphoma tissue, THP-1.

Molecular wt :

Predicted band size: 26 kDa

Subcellular location :

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:500-1:2,000 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-83_4.jpg

Pic legend4 :

Flow cytometric analysis of THP-1 cells labeling Bcl-2. Cells were fixed and permeabilized. Then stained with the primary antibody (EM1701-83, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage