NFIB / NF1B2 | HUABIO EM1701-67 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NFIB / NF1B2

catalog :

EM1701-67

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H2

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NFIB/NF1B2 on SH-SY5Y cell lysates using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.

image 2 :

ICC staining NFIB/NF1B2 (green) in LO-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-NFIB / NF1B2 antibody (EM1701-67) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-67) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1701-67

Target name :

NFIB / NF1B2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human NFIB / NF1B2 aa 1-420 / 420.

Uniprot id :

O00712>SwissProt: O00712 Human;SwissProt: P97863 Mouse;Entrez Gene: 29227 Rat

Host :

Mouse

Clone number :

1H2

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

SH-SY5Y cell lysates, LO-2, rat heart tissue, human colon carcinoma tissue, human prostate tissue, human pancreas tissue, mouse liver tissue, SH-SY-5Y, rat liver tissue, human breast tissue.

Molecular wt :

Predicted band size: 47 kDa

Subcellular location :

Nucleus.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-67_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-NFIB / NF1B2 antibody (EM1701-67) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-67) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1701-67_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-NFIB / NF1B2 antibody (EM1701-67) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-67) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1701-67_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1701-67_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/EM1701-67_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/EM1701-67_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/EM1701-67_10.jpg

Pic legend10 :

Flow cytometric analysis of SH-SY-5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage