product summary
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company name :
HUABIO
product type :
antibody
product name :
PI 3 Kinase p85 alpha
catalog :
EM1701-62
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
L3-D6
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of PI 3 Kinase p85 alpha on different lysates with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of PI 3 Kinase p85 alpha on different lysates with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution.
Lane 1: Raji cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Western blot analysis of PI 3 Kinase p85 alpha on different lysates with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si PI 3 Kinase p85 alpha cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
EM1701-62
Target name :
PI 3 Kinase p85 alpha
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Cell,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human PI3-kinase p85 subunit alpha aa 19-219 / 724.
Uniprot id :
P27986>SwissProt: P27986 Human;SwissProt: P26450 Mouse;SwissProt: Q63787 Rat
Host :
Mouse
Clone number :
L3-D6
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
MCF7 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Raji cell lysate, C6 cell lysate, HepG2, LOVO, human brain tissue, human lung tissue, mouse lung tissue, rat lung tissue.
Molecular wt :
Predicted band size: 84 kDa
Subcellular location :
Cytosol. Endoplasmic reticulum. Golgi apparatus. Nucleus. Plasma Membrane.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IF-Cell: 1:50-1:200
;IHC-P: 1:50-1:1,000
;IF-Tissue: 1:200
Advanced Validation :
Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/EM1701-62_4.jpg
Pic legend4 :
ICC staining PI 3 Kinase p85 alpha (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/EM1701-62_5.jpg
Pic legend5 :
ICC staining PI 3 Kinase p85 alpha (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/EM1701-62_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-62) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/EM1701-62_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded human lung tissue with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/EM1701-62_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/EM1701-62_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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