beta Tubulin | HUABIO EM1701-59 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

beta Tubulin

catalog :

EM1701-59

quantity :

100μl

price :

374.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

1-B11

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (EM1701-59) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-59) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-beta Tubulin antibody (EM1701-59, HRP) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-59, HRP) at 1/2,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-beta Tubulin antibody (EM1701-59, HRP) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-59, HRP) at 1/500 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1701-59

Target name :

beta Tubulin

Species reactivity :

Human,Mouse,Rat,Zebrafish

Applications :

WB,IHC-P

Conjugate :

HRP-conjugated

Immunogen :

Synthetic peptide within Human Beta tubulin aa 46-95 / 444.

Uniprot id :

P07437>SwissProt: P07437 Human;SwissProt: P99024 Mouse;SwissProt: P69897 Rat

Host :

Mouse

Clone number :

1-B11

Isotype :

IgG1

Size :

100μl

List Price :

374.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human colon cancer tissue, human kidney tissue.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Cytoplasm, Cytoskeleton.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IHC-P: 1:500-1:2,000

more info or order :

HUABIO product webpage