Monoamine Oxidase A / MAO-A | HUABIO EM1701-54 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Monoamine Oxidase A / MAO-A

catalog :

EM1701-54

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5D1

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Monoamine Oxidase A / MAO-A on different lysates with Mouse anti-Monoamine Oxidase A / MAO-A antibody (EM1701-54) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: SiHa cell lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 60 kDa Observed band size: 70 kDa Exposure time: Lane 1-2: 46 seconds; Lane 3-4: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining Monoamine Oxidase A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

EM1701-54

Target name :

Monoamine Oxidase A / MAO-A

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human MAO-A aa 222-517 / 527.

Uniprot id :

P21397>SwissProt: P21397 Human;SwissProt: Q64133 Mouse;SwissProt: P21396 Rat

Host :

Mouse

Clone number :

5D1

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, SiHa cell lysate, rat brain tissue lysate, mouse brain tissue lysate, SH-SY-5Y.

Molecular wt :

Predicted band size: 60 kDa

Subcellular location :

Mitochondrion outer membrane.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200

more info or order :

HUABIO product webpage