IFNAR1 | HUABIO EM1701-46 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IFNAR1

catalog :

EM1701-46

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A5-A3

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IFNAR1 on different lysates with Mouse anti-IFNAR1 antibody (EM1701-46) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 64 kDa Observed band size: 64/90/130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-46) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining IFNAR1(red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining IFNAR1(green) in SHG-44 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

EM1701-46

Target name :

IFNAR1

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human IFNAR1 aa 28-127 (Extracellular).

Uniprot id :

P17181>SwissProt: P17181 Human;SwissProt: P33896 Mouse

Host :

Mouse

Clone number :

A5-A3

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, Jurkat cell lysate, NCCIT, SHG-44, human lung tissue, human colon tissue, human skin tissue.

Molecular wt :

Predicted band size: 64 kDa

Subcellular location :

Endosome. Cell membrane.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-46_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1701-46_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1701-46_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage