CD11b | HUABIO EM1701-42 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD11b

catalog :

EM1701-42

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4-6

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1701-42) at 1/1,000 dilution. Lane 1: TF-1 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining CD11b (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining CD11b (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

EM1701-42

Target name :

CD11b

Species reactivity :

Human

Applications :

IHC-P,IF-Cell,FC,WB,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human CD11b aa 544-743 / 1,152.

Uniprot id :

P11215>SwissProt: P11215 Human

Host :

Mouse

Clone number :

4-6

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, A431, LOVO, human tonsil tissue, human spleen tissue.

Molecular wt :

Predicted band size: 127 kDa

Subcellular location :

Cell membrane

Concentration :

2 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC:1:50-1:100 ;WB: 1:1,000 ;IF-Tissue: 1:50

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-42_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1701-42_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11b antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1701-42_6.jpg

Pic legend6 :

Flow cytometric analysis of THP-1 cells with CD11b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1701-42_7.jpg

Pic legend7 :

Application: IF-Tissue Species: Human Site: Spleen Sample: Paraffin-embedded section Antibody concentration: 1/50

more info or order :

HUABIO product webpage