Cardiac Troponin T | HUABIO EM1701-39 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cardiac Troponin T

catalog :

EM1701-39

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3-G6

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cardiac Troponin T on human heart tissue lysate using anti- Cardiac Troponin T antibody at 1/1,000 dilution.

image 2 :

ICC staining Cardiac Troponin T (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining Cardiac Troponin T (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

EM1701-39

Target name :

Cardiac Troponin T

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within huamn Cardiac Troponin T aa 45-89/298.

Uniprot id :

P45379>SwissProt: P45379 Human;SwissProt: P50752 Mouse

Host :

Mouse

Clone number :

3-G6

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human heart tissue lysates, mouse heart tissue, human heart tissue, mouse heart tissue.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Cytoskeleton, Cytosol.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IF-Tissue: 1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-39_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- Cardiac Troponin T antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1701-39_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin T with Mouse anti-Cardiac Troponin T antibody (EM1701-39) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1701-39, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage