AFP | HUABIO EM1701-31 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

AFP

catalog :

EM1701-31

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A5-G3

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of AFP on human placenta tissue lysate using anti-AFP antibody at 1/1,000 dilution.

image 2 :

Western blot analysis of AFP on different lysates with Mouse anti-AFP antibody (EM1701-31) at 1/1,000 dilution. Lane 1: A549-WT cell lysate (10 µg/Lane) Lane 2: A549-KD AFP cell lysate (10 µg/Lane) Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of AFP on different lysates with Mouse anti-AFP antibody (EM1701-31) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-31) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

EM1701-31

Target name :

AFP

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human AFP aa 1-200 / 609.

Uniprot id :

P02771>SwissProt: P02771 Human

Host :

Mouse

Clone number :

A5-G3

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human placenta tissue lysates, A549 cell lysate, Hela cell lysate, HepG2 cell lysate, human liver cancer tissue, human lung cancer tissue, human liver tissue.

Molecular wt :

Predicted band size: 69 kDa

Subcellular location :

Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-31_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1701-31_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1701-31_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AFP antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage