AKR1C1 | HUABIO EM1701-21 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

AKR1C1

catalog :

EM1701-21

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5-F10

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of AKR1C1 on different lysates with Mouse anti-AKR1C1 antibody (EM1701-21) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

image 3 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

product information

SKU :

EM1701-21

Target name :

AKR1C1

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human AKR1C1 aa 180-323 / 323.

Uniprot id :

Q04828>SwissProt: Q04828 Human

Host :

Mouse

Clone number :

5-F10

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HepG2 cell lysate, U-87 MG cell lysate, HeLa cell lysate, A431 cell lysate, human liver tissue lysate, human liver tissue, human pancreas tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 37 kDa

Subcellular location :

Cytoplasm

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-21_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage