TIM 3 | HUABIO EM1701-19-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TIM 3

catalog :

EM1701-19-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E3

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TIM 3 on different lysates with Mouse anti-TIM 3 antibody (EM1701-19) at 1/2,000 dilution. Lane 1: A549 cell lysate (no heat) Lane 2: HepG2 cell lysate (no heat) Lane 3: HDLM-2 cell lysate Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Predicted band size: 33 kDa Observed band size: 42 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-19) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-TIM 3 antibody (EM1701-19) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-TIM 3 antibody (EM1701-19) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1701-19-50UL

Target name :

TIM 3

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein with Human TIM 3 1-89 / 301.

Uniprot id :

Q8TDQ0>SwissProt: Q8TDQ0 Human

Host :

Mouse

Clone number :

1E3

Isotype :

IgG1

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A549 cell lysate, HepG2 cell lysate, HDLM-2 cell lysate, human colon cancer tissue, human tonsil tissue, 293T, Daudi.

Molecular wt :

Predicted band size: 33 kDa

Subcellular location :

Membrane. Cell junction.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:1,000-1:5,000 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-19_4.jpg

Pic legend4 :

Immunocytochemistry analysis of 293T cells labeling Tim3 with Mouse anti-Tim3 antibody (EM1701-19) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Tim3 antibody (EM1701-19) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1701-19_5.jpg

Pic legend5 :

Flow cytometric analysis of Daudi cells with Tim3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage