TIM 3 | HUABIO EM1701-18 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TIM 3

catalog :

EM1701-18

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E6

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of tim3 on 293T cell lysate using anti-Tim3 antibody at 1/100 dilution.

image 2 :

Immunocytochemistry analysis of 293T cells labeling TIM 3 with Mouse anti-TIM 3 antibody (EM1701-18) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-TIM 3 antibody (EM1701-18) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

product information

SKU :

EM1701-18

Target name :

TIM 3

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein with Human TIM 3 1-89 / 301.

Uniprot id :

Q8TDQ0>SwissProt: Q8TDQ0 Human

Host :

Mouse

Clone number :

1E6

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

293T cell lysate, 293T, human tonsil tisssue, human lung cancer tissue, human spleen tissue, Daudi.

Molecular wt :

Predicted band size: 33 kDa

Subcellular location :

Membrane. Cell junction.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2000 ;IF-Cell:1:50-1:200 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-18_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1701-18_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1701-18_6.jpg

Pic legend6 :

Flow cytometric analysis of Daudi cells with Tim3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage