IMP3 | HUABIO EM1701-15-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IMP3

catalog :

EM1701-15-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C2-E4

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1701-15) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Mouse placenta tissue lysate (40 µg/Lane) Lane 4: Rat placenta tissue lysate (40 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-15) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1701-15) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IMP3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-15) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

IMP3 was immunoprecipitated from 0.2 mg 293T cell lysate with EM1701-15 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using EM1701-15 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cell lysate (input) Lane 2: EM1701-15 IP in 293T cell lysate Lane 3: Mouse IgG instead of EM1701-15 in 293T cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds

product information

SKU :

EM1701-15-50UL

Target name :

IMP3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human IMP-3 aa 352-579 / 579.

Uniprot id :

O00425>SwissProt: O00425 Human;SwissProt: Q9CPN8 Mouse;Entrez Gene: 315697 Rat

Host :

Mouse

Clone number :

C2-E4

Isotype :

IgG1

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, 293T cell lysate, mouse placenta tissue lysate, rat placenta tissue lysate, Hela, SH-SY5Y, rat brain tissue, human liver cancer tissue, human placenta tissue, mouse testis tissue.

Molecular wt :

Predicted band size: 64 kDa

Subcellular location :

Cytoplasm. Nucleus.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:50 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: 1-2μg/sample

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1701-15_4.jpg

Pic legend4 :

ICC staining IMP3 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1701-15_5.jpg

Pic legend5 :

ICC staining IMP3 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1701-15_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1701-15_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/EM1701-15_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/EM1701-15_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMP3 antibody. Counter stained with hematoxylin.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/EM1701-15_10.jpg

Pic legend10 :

Flow cytometric analysis of HUVEC cells with IMP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage