Alpha 1 Acid Glycoprotein | HUABIO EM1510-42 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Alpha 1 Acid Glycoprotein

catalog :

EM1510-42

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A4-G2-A3

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Alpha 1 Acid Glycoprotein on different lysates with Mouse anti-Alpha 1 Acid Glycoprotein antibody (EM1510-42) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: THP-1 cell lysate (20 µg/Lane) Lane 4: HCT 116 cell lysate (20 µg/Lane) Lane 5: 293T cell lysate (20 µg/Lane) Lane 6: NIH:OVCAR-3 cell lysate (20 µg/Lane) Lane 7: Human liver tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 37/45 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1510-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.

image 3 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.

product information

SKU :

EM1510-42

Target name :

Alpha 1 Acid Glycoprotein

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ORM1 aa 1-201 / 201.

Uniprot id :

P02763>SwissProt: P02763 Human;SwissProt: Q60590 Mouse;SwissProt: P02764 Rat

Host :

Mouse

Clone number :

A4-G2-A3

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HepG2 cell lysate, HeLa cell lysate, THP-1 cell lysate, HCT 116 cell lysate, 293T cell lysate, NIH:OVCAR-3 cell lysate, human liver tissue lysate, rat liver tissue, human lung cancer tissue, human esophagus tissue, HepG2.

Molecular wt :

Predicted band size: 24 kDa

Subcellular location :

Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:50-1:100 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1510-42_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1510-42_5.jpg

Pic legend5 :

Flow cytometric analysis of HepG2 cells with Alpha 1 Acid Glycoprotein antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-Mouse IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage