BAX | HUABIO EM1203 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BAX

catalog :

EM1203

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3-D2

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BAX on different lysates with Mouse anti-BAX antibody (EM1203) at 1/500 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1203) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining of BAX in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1203, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Flow cytometric analysis of Hela cells with vimentin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgA (FITC) was used as the secondary antibody.

product information

SKU :

EM1203

Target name :

BAX

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to N-terminal BAX.

Uniprot id :

Q07812>SwissProt: Q07812 Human;SwissProt: Q07813 Mouse

Host :

Mouse

Clone number :

3-D2

Isotype :

IgA

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Daudi cell lysate, Raji cell lysate, HepG2, Hela.

Molecular wt :

Predicted band size: 21 kDa

Subcellular location :

Mitochondrion membrane, cytoplasm

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500;IF-Cell: 1:200;IHC-P: 1:200;FC: 1:50

more info or order :

HUABIO product webpage