EpCAM | HUABIO EM1111 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

EpCAM

catalog :

EM1111

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3-E1-E2

reactivity :

human, mouse

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of EpCAM on different lysates using anti- EpCAM antibody at 1/1000 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: MCF-7 cell lysate

image 2 :

ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

EM1111

Target name :

EpCAM

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human EP-CAM aa 24-265 (Extracellular).

Uniprot id :

P16422>SwissProt: P16422 Human;SwissProt: Q99JW5 Mouse

Host :

Mouse

Clone number :

3-E1-E2

Isotype :

IgM

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein L affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Hela cell lysate, NIH/3T3 cell lysate, MCF-7 cell lysate, D3, A431.

Molecular wt :

Predicted band size: 35 kDa

Subcellular location :

Cell membrane

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1111_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells labeling EpCAM with Mouse anti-EpCAM antibody (EM1111) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-EpCAM antibody (EM1111) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage