Oct4 | HUABIO EM100306 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Oct4

catalog :

EM100306

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A3-C11

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Oct4 on different lysates with Mouse anti-Oct4 antibody (EM100306) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Oct4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 42 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM100306) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Oct4 on different lysates using anti-Oct4 antibody at 1/1,000 dilution. Positive control: Lane 1: NCCIT cell lysate Lane 2: D3 cell lysate

image 3 :

ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

EM100306

Target name :

Oct4

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within full length human Oct4 protein.

Uniprot id :

Q01860>SwissProt: Q01860 Human

Host :

Mouse

Clone number :

A3-C11

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

NCCIT cell lysate, D3 cell lysate, NCCIT.

Molecular wt :

Predicted band size: 39 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:200 ;FC: 1:1000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM100306_4.jpg

Pic legend4 :

Flow cytometric analysis of NCCIT cells labeling Oct4. Cells were fixed and permeabilized. Then stained with the primary antibody (EM100306, 1:1000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage