CD44 | HUABIO EM010805 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD44

catalog :

EM010805

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A7-1

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD44 on different lysates with Mouse anti-CD44 antibody (EM010805) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HUVEC cell lysate Lane 3: PANC-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 4 Seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM010805) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CD44 on different lysates using anti- CD44 antibody at 1/500 dilution. Positive control: Lane 1: HUVEC cell lysate Lane 2: HL-60 cell lysate Lane 3: Hela cell lysate Lane 4: SKOV-3 cell lysate

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD44 antibody. Counter stained with hematoxylin.

product information

SKU :

EM010805

Target name :

CD44

Species reactivity :

Human

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human CD44 aa 100-200.

Uniprot id :

P16070>SwissProt: P16070 Human

Host :

Mouse

Clone number :

A7-1

Isotype :

IgM

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HUVEC cell lysate, HL-60 cell lysate, Hela cell lysate, SKOV-3 cell lysate, human tonsil tissue, human colon carcinoma tissue, Hela.

Molecular wt :

Predicted band size: 81 kDa

Subcellular location :

Cell membrane

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:200 ;FC: 1:100-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM010805_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD44 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM010805_5.jpg

Pic legend5 :

Flow cytometric analysis of Hela cells with CD44 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage