HUABIO E-cadherin antibody

E-Cadherin
HUABIO
catalog: 0407-25

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, flow cytometry

Western blot analysis of E-Cadherin on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

ICC staining E-Cadherin in HCT116 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-25) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

ICC staining E-Cadherin in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-25) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
quantity: 100μl
price: 330 USD
to the supplier

E-Cadherin
HUABIO
catalog: EM0502

mouse monoclonal (A0-G11-2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of E-Cadherin with anti-E-Cadherin antibody [A0-G11-2] (EM0502) at 1:1,000 dilution. Lane 1/2: Wild-type A431 whole cell lysate (20 µg). Lane 3/4: E-Cadherin fragment 1 knockdown A431 whole cell lysate (20 µg). Lane 5/6: E-Cadherin fragment 2 knockdown A431 whole cell lysate (20 µg). Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM0502, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 1 hour at room temperature.

Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (EM0502) at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 98 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0502) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-E-Cadherin antibody (EM0502) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

E-Cadherin
HUABIO
catalog: ER63312

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Colon Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Colon Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (ER63312) at 1/10,000 dilution. Lane 1: T-47D cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (negative) (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: 4T1 cell lysate (20 µg/Lane) Lane 5: C2C12 cell lysate (negative) (20 µg/Lane) Lane 6: Mouse pancreas tissue lysate (20 µg/Lane) Lane 7: Rat pancreas tissue lysate (20 µg/Lane) Predicted band size: 97 kDa Observed band size: 80-130 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63312) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

E-Cadherin
HUABIO
catalog: ET1607-75

domestic rabbit monoclonal (SY0287)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (ET1607-75) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (ET1607-75) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (ET1607-75) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: HT-29 cell lysate Lane 4: HCT 116 cell lysate Lane 5: A431 cell lysate Lane 6: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 80~120 kDa Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-75) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of E-Cadherin with anti-E-Cadherin antibody (ET1607-75) at 1/5,000 dilution. Lane 1: Wild-type A431 whole cell lysate (10 µg). Lane 2/3: E-Cadherin knockdown A431 whole cell lysate (10 µg). Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1607-75, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Pan-Cadherin
HUABIO
catalog: ET1609-70-50UL

domestic rabbit monoclonal (ST54-01)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Pan-Cadherin on different lysates with Rabbit anti-Pan-Cadherin antibody (ET1609-70) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: Mouse embryo tissue lysate (40 µg/Lane) Lane 4: Mouse placenta tissue lysate (40 µg/Lane) Lane 5: Rat embryo tissue lysate (40 µg/Lane) Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-70) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Pan-Cadherin on different lysates with Rabbit anti-Pan-Cadherin antibody (ET1609-70) at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-70) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling Pan-Cadherin with Rabbit anti-Pan-Cadherin antibody (ET1609-70) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pan-Cadherin antibody (ET1609-70) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 50μl
price: 205.00 USD
to the supplier

E-Cadherin
HUABIO
catalog: HA601143

mouse monoclonal (A0-G11-2-R)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (HA601143) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 98 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601143) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

Fluorescence multiplex immunohistochemical analysis of human kidney (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD31 (M1511-8, Red), anti-E-Cadherin (HA601143, Green), anti-Calbindin (ET1702-54, Magenta) on human kidney. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of M1511-8 (1/1,000 dilution), HA601143 (1/4,000 dilution) and ET1702-54 (1/4,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Fluorescence multiplex immunohistochemical analysis of mouse small intestine (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-E-Cadherin (HA601143, Red) and anti-Lysozyme (ET1609-35, Green) on small intestine. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in two rounds of staining: in the order of HA601143 (1/4,000 dilution) and ET1609-35 (1/2,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
quantity: 100μl
price: 360.00 USD
to the supplier

E-Cadherin
HUABIO
catalog: HA610047

mouse monoclonal (A0-G11-2-R)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (HA610047) at 1/1,000 dilution. Lane 1: A431 cell lysate (10 µg/Lane) Lane 2: SW480 cell lysate (10 µg/Lane) Lane 3: MCF7 cell lysate (10 µg/Lane) Predicted band size: 98 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610047) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-E-Cadherin antibody (HA610047) at 1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610047) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-E-Cadherin antibody (HA610047) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610047) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier

E-Cadherin
HUABIO
catalog: HA720159F

domestic rabbit monoclonal (SY0287)
reactivity: human
application: flow cytometry

Immunocytochemistry analysis of MCF-7 cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

Flow cytometric analysis of A431 cells labeling E-Cadherin. Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of MCF-7 cells labeling E-Cadherin. Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 10ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 10ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 429.00 USD
to the supplier

E-Cadherin
HUABIO
catalog: HA720164F

domestic rabbit monoclonal (SY0287)
reactivity: human

Immunofluorescence analysis of paraffin-embedded human breast carcinoma tissue labeling E-Cadherin (HA720164F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody E-Cadherin (HA720164F, iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
quantity: 100μl
price: 429.00 USD
to the supplier

Human E-Cadherin
HUABIO
catalog: HA723055

domestic rabbit monoclonal (PSH09-02)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human E-Cadherin matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723055) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human E-Cadherin protein (HA210886) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723056, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native E-Cadherin in Jurkat and MCF-7 cell culture supernatant. The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are MCF-7 cell culture supernatant 50% and Jurkat cell culture supernatnat 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was undetectable in Jurkat cell culture supernatant and 13.7 ng/ml in MCF-7 cell culture supernatant.

Interpolated concentrations of native E-Cadherin in human urine and human serum samples. The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are human urine 13% and human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 33.1 ng/ml in human urine and 137.0 ng/ml in human serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Human E-Cadherin
HUABIO
catalog: HA723056

domestic rabbit monoclonal (SY0287)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human E-Cadherin matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723055) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human E-Cadherin protein (HA210886) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723056, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native E-Cadherin in Jurkat and MCF-7 cell culture supernatant. The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are MCF-7 cell culture supernatant 50% and Jurkat cell culture supernatnat 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was undetectable in Jurkat cell culture supernatant and 13.7 ng/ml in MCF-7 cell culture supernatant.

Interpolated concentrations of native E-Cadherin in human urine and human serum samples. The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are human urine 13% and human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 33.1 ng/ml in human urine and 137.0 ng/ml in human serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Human E-Cadherin
HUABIO
catalog: HA723057B

domestic rabbit monoclonal (SY0287)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human E-Cadherin matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723055) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human E-Cadherin protein (HA210886) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723057B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native E-Cadherin in Jurkat and MCF-7 cell culture supernatant. The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are MCF-7 cell culture supernatant 50% and Jurkat cell culture supernatnat 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was undetectable in Jurkat cell culture supernatant and 13.7 ng/ml in MCF-7 cell culture supernatant.

Interpolated concentrations of native E-Cadherin in human urine and human serum samples. The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are human urine 13% and human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 33.1 ng/ml in human urine and 137.0 ng/ml in human serum.
quantity: 100μl
price: 409.00 USD
to the supplier

E-Cadherin
HUABIO
catalog: HA723564

domestic rabbit monoclonal (PSH13-75)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (HA723564) at 1/5,000 dilution. Lane 1: 4T1 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (negative) (20 µg/Lane) Lane 3: Mouse small intestine tissue lysate (30 µg/Lane) Lane 4: Mouse colon tissue lysate (30 µg/Lane) Lane 5: Mouse pancreas tissue lysate (30 µg/Lane) Lane 6: Rat pancreas tissue lysate (30 µg/Lane) Lane 7: Rat lung tissue lysate (30 µg/Lane) Lane 8: Rat colon tissue lysate (30 µg/Lane) Lane 9: MCF7 cell lysate (20 µg/Lane) Lane 10: MDA-MB-231 cell lysate (negative) (20 µg/Lane) Lane 11: HT-29 cell lysate (20 µg/Lane) Lane 12: Caco-2 cell lysate (20 µg/Lane) Predicted band size: 98 kDa Observed band size: 75-130 kDa Exposure time: Lane 1-5: 10 seconds; Lane 3: 1 minute 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723564) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: colon Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: colon Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

E-Cadherin
HUABIO
catalog: HA750133

domestic rabbit monoclonal (SY0287)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (HA750133) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (HA750133) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (HA750133) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: HT-29 cell lysate Lane 4: HCT 116 cell lysate Lane 5: A431 cell lysate Lane 6: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 80~120 kDa Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750133) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of E-Cadherin with anti-E-Cadherin antibody (HA750133) at 1/5,000 dilution. Lane 1: Wild-type A431 whole cell lysate (10 µg). Lane 2/3: E-Cadherin knockdown A431 whole cell lysate (10 µg). Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA750133, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Pan-Cadherin
HUABIO
catalog: HA750190

domestic rabbit monoclonal (ST54-01)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Pan-Cadherin on different lysates with Rabbit anti-Pan-Cadherin antibody (HA750190) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: Mouse embryo tissue lysate (40 µg/Lane) Lane 4: Mouse placenta tissue lysate (40 µg/Lane) Lane 5: Rat embryo tissue lysate (40 µg/Lane) Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750190) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Pan-Cadherin on different lysates with Rabbit anti-Pan-Cadherin antibody (HA750190) at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750190) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling Pan-Cadherin with Rabbit anti-Pan-Cadherin antibody (HA750190) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pan-Cadherin antibody (HA750190) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier

E-Cadherin
HUABIO
catalog: HA751488

domestic rabbit monoclonal (PSH13-75)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section