CD48
HUABIO
catalog: HA721371
domestic rabbit monoclonal (JE37-98)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA721371) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: THP-1 cell lysate (negative) Lane 4: K-562 cell lysate (negative) Lane 5: Ramos cell lysate Lane 6: Daudi cell lysate Lane 7: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 40-50 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721371) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD48 antibody (HA721371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD48 antibody (HA721371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Human CD48
HUABIO
catalog: HA722977
domestic rabbit monoclonal (PSH08-36)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD48 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722977) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD48 protein (HA210906) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722978, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD48 in human cell culture supernatant samples. Interpolated concentration of native CD48 was measured in duplicate at different sample concentrations and interpolated from the CD48 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD48 concentration was determined to be 329 pg/mL in Daudi and 237 pg/mL in Ramos in cell culture supernatant. There was no detectable signal in Hela and K-562 cell supernatant.

Interpolated concentrations of spiked CD48 in cell culture media samples. The concentrations of CD48 were measured in duplicates, interpolated from the CD48 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD48
HUABIO
catalog: HA722978
domestic rabbit monoclonal (PSH08-37)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD48 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722977) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD48 protein (HA210906) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722978, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of human CD48 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722978) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD48 protein (HA210906) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722979, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD48 in human cell culture supernatant samples. Interpolated concentration of native CD48 was measured in duplicate at different sample concentrations and interpolated from the CD48 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD48 concentration was determined to be 329 pg/mL in Daudi and 237 pg/mL in Ramos in cell culture supernatant. There was no detectable signal in Hela and K-562 cell supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD48
HUABIO
catalog: HA722979
domestic rabbit monoclonal (PSH08-38)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD48 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722978) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD48 protein (HA210906) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722979, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD48 in human cell culture supernatant samples. Interpolated concentration of native CD48 was measured in duplicate at different sample concentrations and interpolated from the CD48 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD48 concentration was determined to be 405 pg/mL in Daudi and 269 pg/mL in Ramos cell culture supernatant. There was no detectable signal in Hela and K-562 cell supernatant.

Interpolated concentrations of spiked CD48 in cell culture media samples. The concentrations of CD48 were measured in duplicates, interpolated from the CD48 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD48
HUABIO
catalog: HA722980B
domestic rabbit monoclonal (PSH08-37)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CD48 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722977) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD48 protein (HA210906) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722980B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD48 in human cell culture supernatant samples. Interpolated concentration of native CD48 was measured in duplicate at different sample concentrations and interpolated from the CD48 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD48 concentration was determined to be 329 pg/mL in Daudi and 237 pg/mL in Ramos in cell culture supernatant. There was no detectable signal in Hela and K-562 cell supernatant.

Interpolated concentrations of spiked CD48 in cell culture media samples. The concentrations of CD48 were measured in duplicates, interpolated from the CD48 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Human CD48
HUABIO
catalog: HA722981B
domestic rabbit monoclonal (PSH08-38)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CD48 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722978) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD48 protein (HA210906) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722981B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD48 in human cell culture supernatant samples. Interpolated concentration of native CD48 was measured in duplicate at different sample concentrations and interpolated from the CD48 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD48 concentration was determined to be 405 pg/mL in Daudi and 269 pg/mL in Ramos cell culture supernatant. There was no detectable signal in Hela and K-562 cell supernatant.

Interpolated concentrations of spiked CD48 in cell culture media samples. The concentrations of CD48 were measured in duplicates, interpolated from the CD48 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
CD48
HUABIO
catalog: HA723230
domestic rabbit monoclonal (PSH10-65)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA723230) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: Ramos cell lysate Lane 4: THP-1 cell lysate (negative) Lane 5: Daudi cell lysate Lane 6: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 40-50 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723230) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of HeLa (left, negative) and Daudi (right, positive) cells labeling CD48. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723230, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of THP-1 (left, negative) and Daudi (right, positive) cells labeling CD48. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723230, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
CD48
HUABIO
catalog: HA751368
domestic rabbit monoclonal (PSH10-65)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA751368) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: Ramos cell lysate Lane 4: THP-1 cell lysate (negative) Lane 5: Daudi cell lysate Lane 6: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 40-50 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751368) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of HeLa (left, negative) and Daudi (right, positive) cells labeling CD48. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751368, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of THP-1 (left, negative) and Daudi (right, positive) cells labeling CD48. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751368, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier