CD47
HUABIO
catalog: EM1701-38

mouse monoclonal (1A9)
reactivity: human
application: flow cytometry, immunohistochemistry - paraffin section

ICC staining CD47 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Mouse anti-CD47 antibody (EM1701-38) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-38) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-CD47 antibody (EM1701-38) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

CD47
HUABIO
catalog: HA600104F

mouse monoclonal (1B1)
reactivity: human
application: flow cytometry

Flow cytometric analysis of MCF-7 cells labeling CD47. Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with HA600104F CD47 (HA600104F, red, 1ug/ml) and Mouse IgG1 Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 394.00 USD
to the supplier

CD47
HUABIO
catalog: HA721158

domestic rabbit monoclonal (PD00-33)
reactivity: human
application: flow cytometry, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (HA721158) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721158) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-CD47 antibody (HA721158) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721158) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of Jurkat cells labeling CD47. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721158, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

CD47
HUABIO
catalog: HA723198

domestic rabbit monoclonal (PSH10-31)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD47 on different lysates with Rabbit anti-CD47 antibody (HA723198) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HDLM-2 cell lysate Lane 3: HepG2 cell lysate (negative) Lane 4: U-937 cell lysate Lane 5: A549 cell lysate Lane 6: NIH:OVCAR-3 cell lysate Lane 7: MOLT-4 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 45-55 kDa Exposure time: Lane 1-5: 3 minutes; Lane 6-7: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723198) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Jurkat (positive) and HepG2 (negative) labeling CD47 with Rabbit anti-CD47 antibody (HA723198) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD47 antibody (HA723198) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HepG2 (left, negative) and Jurkat (right, positive) cells labeling CD47. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723198, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

Human CD47
HUABIO
catalog: HA723272

domestic rabbit monoclonal (PSH11-05)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD47 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723272) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD47 protein (HA211050) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723273, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD47 in Jurkat and HepG2 extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD47 was measured in duplicate at different sample concentrations and interpolated from the CD47 standard curves. The mean CD47 concentration was determined to be 1,853 pg/mL in Jurkat cell extract, There was no detectable signal in HepG2 cell extract.

Interpolated concentrations of spiked CD47 in cell culture media samples. The concentrations of CD47 were measured in duplicates, interpolated from the CD47 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human CD47
HUABIO
catalog: HA723273

domestic rabbit monoclonal (PSH11-06)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD47 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723272) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD47 protein (HA211050) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723273, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD47 in Jurkat and HepG2 extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD47 was measured in duplicate at different sample concentrations and interpolated from the CD47 standard curves. The mean CD47 concentration was determined to be 1,853 pg/mL in Jurkat cell extract, There was no detectable signal in HepG2 cell extract.

Interpolated concentrations of spiked CD47 in cell culture media samples. The concentrations of CD47 were measured in duplicates, interpolated from the CD47 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human CD47
HUABIO
catalog: HA723274B

domestic rabbit monoclonal (PSH11-06)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CD47 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723272) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD47 protein (HA211050) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723273, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD47 in Jurkat and HepG2 extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD47 was measured in duplicate at different sample concentrations and interpolated from the CD47 standard curves. The mean CD47 concentration was determined to be 1,853 pg/mL in Jurkat cell extract, There was no detectable signal in HepG2 cell extract.

Interpolated concentrations of spiked CD47 in cell culture media samples. The concentrations of CD47 were measured in duplicates, interpolated from the CD47 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

CD47
HUABIO
catalog: HA751348

domestic rabbit monoclonal (PSH10-31)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD47 on different lysates with Rabbit anti-CD47 antibody (HA751348) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HDLM-2 cell lysate Lane 3: HepG2 cell lysate (negative) Lane 4: U-937 cell lysate Lane 5: A549 cell lysate Lane 6: NIH:OVCAR-3 cell lysate Lane 7: MOLT-4 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 45-55 kDa Exposure time: Lane 1-5: 3 minutes; Lane 6-7: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751348) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Jurkat (positive) and HepG2 (negative) labeling CD47 with Rabbit anti-CD47 antibody (HA751348) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD47 antibody (HA751348) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HepG2 (left, negative) and Jurkat (right, positive) cells labeling CD47. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751348, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier