mouse monoclonal (7G1)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of PRDX6 on PC-3M (1) and K562 (2) using anti-PRDX6 antibody at 1/500 dilution.

ICC staining PRDX6 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining PRDX6 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit monoclonal (JM11-13)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody (ET1703-62) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 25 kDa Observed band size: 27 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody (ET1703-62) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Peroxiredoxin 6 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 27 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-62) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
mouse monoclonal (7G1-R)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (HA601301) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 25 kDa Observed band size: 27 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601301) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (HA601301) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Peroxiredoxin 6 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 27 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601301) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
mouse monoclonal (7G1-R)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (HA610174) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 25 kDa Observed band size: 27 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610174) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (HA610174) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Peroxiredoxin 6 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 27 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610174) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μg
price: 649.00 USD
to the supplier
