GDF15
HUABIO
catalog: HA722551

domestic rabbit monoclonal (JE39-90)
reactivity: human
application: western blot

Western blot analysis of GDF15 on different lysates with Rabbit anti-GDF15 antibody (HA722551) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: LNCaP cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34/12.5 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722551) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

GDF15
HUABIO
catalog: HA723178

domestic rabbit monoclonal (PSH10-09)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of GDF15 on different lysates with Rabbit anti-GDF15 antibody (HA723178) at 1/2,000 dilution. Lane 1: LNCaP cell lysate Lane 2: K-562 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 34 kDa Observed band size: 35/15 kDa Exposure time: 1 minute 33 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723178) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GDF15 antibody (HA723178) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723178) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-GDF15 antibody (HA723178) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723178) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Human GDF-15
HUABIO
catalog: HA723805

domestic rabbit monoclonal (PSH16-00)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human GDF15 matched pair antibodies Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723806, Human GDF-15 Rabbit mAb [PSH16-01] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723805) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GDF-15 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA723806, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human GDF15 matched pair antibodies Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-02] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723805) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GDF-15 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA723807, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native GDF-15 in human samples. Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-01] Interpolated concentration of native GDF-15 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 17,760 pg/mL in HepG2 and 1,208 pg/ml in JAR cell culture supernatant . There was no detectable signal in U-937 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human GDF-15
HUABIO
catalog: HA723806

domestic rabbit monoclonal (PSH16-01)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human GDF15 matched pair antibodies Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723806, Human GDF-15 Rabbit mAb [PSH16-01] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723805) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GDF-15 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA723806, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native GDF-15 in human samples. Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-01] Interpolated concentration of native GDF-15 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 17,760 pg/mL in HepG2 and 1,208 pg/ml in JAR cell culture supernatant . There was no detectable signal in U-937 cell culture supernatant.

Interpolated concentrations of spiked GDF-15 in cell culture media samples. Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-01] The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human GDF-15
HUABIO
catalog: HA723806B

domestic rabbit monoclonal (PSH16-01)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human GDF15 matched pair antibodies Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723806, Human GDF-15 Rabbit mAb [PSH16-01] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723805) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GDF-15 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA723806, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native GDF-15 in human samples. Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-01] Interpolated concentration of native GDF-15 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 17,760 pg/mL in HepG2 and 1,208 pg/ml in JAR cell culture supernatant . There was no detectable signal in U-937 cell culture supernatant.

Interpolated concentrations of spiked GDF-15 in cell culture media samples. Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-01] The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

Human GDF-15
HUABIO
catalog: HA723807

domestic rabbit monoclonal (PSH16-02)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human GDF15 matched pair antibodies Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-02] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723805) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GDF-15 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA723807, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native GDF-15 in human samples. Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-02] Interpolated concentration of native GDF-15 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 21,440 pg/mL in HepG2 and 1,319 pg/ml in JAR cell culture supernatant . There was no detectable signal in U-937 cell culture supernatant.

Interpolated concentrations of spiked GDF-15 in cell culture media samples. Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-02] The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human GDF-15
HUABIO
catalog: HA723807B

domestic rabbit monoclonal (PSH16-02)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human GDF15 matched pair antibodies Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-02] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723805) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GDF-15 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA723807, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native GDF-15 in human samples. Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-02] Interpolated concentration of native GDF-15 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 21,440 pg/mL in HepG2 and 1,319 pg/ml in JAR cell culture supernatant . There was no detectable signal in U-937 cell culture supernatant.

Interpolated concentrations of spiked GDF-15 in cell culture media samples. Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00] Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-02] The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

GDF15
HUABIO
catalog: HA751339

domestic rabbit monoclonal (PSH10-09)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of GDF15 on different lysates with Rabbit anti-GDF15 antibody (HA751339) at 1/2,000 dilution. Lane 1: LNCaP cell lysate Lane 2: K-562 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 34 kDa Observed band size: 35/15 kDa Exposure time: 1 minute 33 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751339) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GDF15 antibody (HA751339) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751339) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-GDF15 antibody (HA751339) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751339) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier