Human CD33
HUABIO
catalog: HA720197F
domestic rabbit monoclonal (PSH04-96)
reactivity: human
conjugate: FITC
application: flow cytometry

Flow cytometric analysis of human peripheral blood labelling Human CD33 (HA720197F, FITC).
quantity: 100μl
price: 429.00 USD
to the supplier
CD33
HUABIO
catalog: HA721826
domestic rabbit monoclonal (PSH02-50)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD33 on different lysates with Rabbit anti-CD33 antibody (HA721826) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721826) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD33 on different lysates with Rabbit anti-CD33 antibody (HA721826) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 cell lysate treated with deglycosylation Predicted band size: 40 kDa Observed band size: 40/70 kDa (Glycosylated) Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721826) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling CD33 with Rabbit anti-CD33 antibody (HA721826) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD33 antibody (HA721826) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
CD33
HUABIO
catalog: HA721827
domestic rabbit monoclonal (PSH02-51)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD33 on different lysates with Rabbit anti-CD33 antibody (HA721827) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling CD33 with Rabbit anti-CD33 antibody (HA721827) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD33 antibody (HA721827) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD33 with Rabbit anti-CD33 antibody (HA721827) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD33 antibody (HA721827) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
Human CD33
HUABIO
catalog: HA722254
domestic rabbit monoclonal (PSH05-25)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CD33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722254) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD33 protein (HA210949) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA722255, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

The concentrations of CD33 were interpolated from the CD33 standard curves and corrected for sample dilution. Undiluted samples are mixed serum from twenty-one volunteers 5%. The mean CD33 concentration was determined to be 7,466 pg/mL in mixed serum from twenty-one volunteers.

Interpolated concentrations of native CD33 in U937 and HL-60 extract samples based on a 1,000 µg/mL extract load. The concentrations of CD33 were measured in triplicates, interpolated from the CD33 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 5% and HL-60 extract 1%. The mean CD33 concentration was determined to be 5,333 pg/mL in U937 extract and 21,225 pg/mL in HL-60 extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD33
HUABIO
catalog: HA722255
domestic rabbit monoclonal (PSH05-26)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CD33 matched pair antibodie Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722254) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD33 protein (HA210949) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA722255, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

The concentrations of CD33 were interpolated from the CD33 standard curves and corrected for sample dilution. Undiluted samples are mixed serum from twenty-one volunteers 5%. The mean CD33 concentration was determined to be 7,466 pg/mL in mixed serum from twenty-one volunteers.

Interpolated concentrations of native CD33 in U937 and HL-60 extract samples based on a 1,000 µg/mL extract load. The concentrations of CD33 were measured in triplicates, interpolated from the CD33 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 5% and HL-60 extract 1%. The mean CD33 concentration was determined to be 5,333 pg/mL in U937 extract and 21,225 pg/mL in HL-60 extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD33
HUABIO
catalog: HA722599B
domestic rabbit monoclonal (PSH05-26)
reactivity: human
conjugate: biotin
application: ELISA
quantity: 100μl
price: 409.00 USD
to the supplier
CD33
HUABIO
catalog: HA750813
domestic rabbit monoclonal (PSH02-50)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD33 on different lysates with Rabbit anti-CD33 antibody (HA750813) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750813) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD33 on different lysates with Rabbit anti-CD33 antibody (HA750813) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 cell lysate treated with deglycosylation Predicted band size: 40 kDa Observed band size: 40/70 kDa (Glycosylated) Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling CD33 with Rabbit anti-CD33 antibody (HA750813) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD33 antibody (HA750813) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
CD33
HUABIO
catalog: HA750814
domestic rabbit monoclonal (PSH02-51)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD33 on different lysates with Rabbit anti-CD33 antibody (HA750814) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750814) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling CD33 with Rabbit anti-CD33 antibody (HA750814) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD33 antibody (HA750814) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD33 with Rabbit anti-CD33 antibody (HA750814) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD33 antibody (HA750814) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier