CD80
HUABIO
catalog: 1007-8
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of CD80 on Raji cell lysates with Rabbit anti-CD80 antibody (1007-8) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 55-75 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1007-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Raji cells labeling CD80 with Rabbit anti-CD80 antibody (1007-8) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD80 antibody (1007-8) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier
CD80
HUABIO
catalog: ET1702-95
domestic rabbit monoclonal (JF100-4)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD80 on different lysates with Rabbit anti-CD80 antibody (ET1702-95) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 60-80 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-95) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
CD80
HUABIO
catalog: HA601247-50UL
mouse monoclonal (8-E5-R)
reactivity: human
application: western blot

Western blot analysis of CD80 on human brain tissue lysates with Mouse anti-CD80 antibody (HA601247) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 33 kDa Observed band size: 60 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 50μl
price: 205.00 USD
to the supplier
CD80
HUABIO
catalog: HA610122
mouse monoclonal (8-E5-R)
reactivity: human
application: western blot

Western blot analysis of CD80 on human brain tissue lysates with Mouse anti-CD80 antibody (HA610122) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 33 kDa Observed band size: 60 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610122) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μg
price: 649.00 USD
to the supplier
Human CD80
HUABIO
catalog: HA723123
domestic rabbit monoclonal (PSH09-61)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD80 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723123) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD80 protein (HA210994) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723124, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of human CD80 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723123) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD80 protein (HA210994) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723125, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD80 in Raji and HUVEC extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD80 was measured in duplicate at different sample concentrations and interpolated from the CD80 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 30,943 pg/mL in Raji cell extract, There was no detectable signal in HUVEC cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD80
HUABIO
catalog: HA723124
domestic rabbit monoclonal (PSH09-62)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD80 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723123) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD80 protein (HA210994) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723124, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD80 in Raji and HUVEC extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD80 was measured in duplicate at different sample concentrations and interpolated from the CD80 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 30,943 pg/mL in Raji cell extract, There was no detectable signal in HUVEC cell extract.

Interpolated concentrations of spiked CD80 in cell culture media samples. The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD80
HUABIO
catalog: HA723125
domestic rabbit monoclonal (PSH09-63)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD80 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723123) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD80 protein (HA210994) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723125, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD80 in Raji and HUVEC extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD80 was measured in duplicate at different sample concentrations and interpolated from the CD80 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 40,639 pg/mL in Raji cell extract, There was no detectable signal in HUVEC cell extract.

Interpolated concentrations of spiked CD80 in cell culture media samples. The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD80
HUABIO
catalog: HA723126B
domestic rabbit monoclonal (PSH09-62)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CD80 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723123) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD80 protein (HA210994) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723126B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD80 in Raji and HUVEC extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD80 was measured in duplicate at different sample concentrations and interpolated from the CD80 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 30,943 pg/mL in Raji cell extract, There was no detectable signal in HUVEC cell extract.

Interpolated concentrations of spiked CD80 in cell culture media samples. The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Human CD80
HUABIO
catalog: HA723127B
domestic rabbit monoclonal (PSH09-63)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CD80 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723123) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD80 protein (HA210994) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723127B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD80 in Raji and HUVEC extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD80 was measured in duplicate at different sample concentrations and interpolated from the CD80 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 40,639 pg/mL in Raji cell extract, There was no detectable signal in HUVEC cell extract.

Interpolated concentrations of spiked CD80 in cell culture media samples. The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
CD80
HUABIO
catalog: HA723144
domestic rabbit monoclonal (PSH09-79)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD80 on different lysates with Rabbit anti-CD80 antibody (HA723144) at 1/2,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (negative) (20 µg/Lane) Lane 3: HDLM-2 cell lysate (20 µg/Lane) Predicted band size: 33 kDa Observed band size: 55-60 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723144) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Raji (positive) and 293T (negative) labeling CD80 with Rabbit anti-CD80 antibody (HA723144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD80 antibody (HA723144) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of 293T (left, negative) and Raji (right, positive) cells labeling CD80. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723144, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
CD80
HUABIO
catalog: HA751321
domestic rabbit monoclonal (PSH09-79)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD80 on different lysates with Rabbit anti-CD80 antibody (HA751321) at 1/2,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (negative) (20 µg/Lane) Lane 3: HDLM-2 cell lysate (20 µg/Lane) Predicted band size: 33 kDa Observed band size: 55-60 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751321) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Raji (positive) and 293T (negative) labeling CD80 with Rabbit anti-CD80 antibody (HA751321) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD80 antibody (HA751321) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of 293T (left, negative) and Raji (right, positive) cells labeling CD80. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751321, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier
CD80
HUABIO
catalog: M1007-10
mouse monoclonal (8-E5)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD80 on human brain tissue lysates with Mouse anti-CD80 antibody (M1007-10) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 33 kDa Observed band size: 60 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1007-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin- embedded human brain tissue using using anti-CD80 Mouse mAb (Cat. # M1007-10).

Flow cytometric analysis of HUVEC (left, negative) and Raji (right, positive) cells labeling CD80. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (M1007-10, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 360.00 USD
to the supplier