domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of CD28 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysate Lane 2: U937 cell lysate
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD28 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-42, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human tonsils tissue using anti-CD28 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-42, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH12-50)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human CD28 matched pair antibodies Capture: HA723457, Human CD28 Rabbit mAb [PSH12-50] Detector: HA723458, Human CD28 Rabbit mAb [PSH12-51] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723457) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD28 protein (HA210920) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723458, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CD28 in human samples. Capture: HA723457, Human CD28 Rabbit mAb [PSH12-50] Detector: HA723458, Human CD28 Rabbit mAb [PSH12-51] Interpolated concentration of native CD28 was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The mean CD28 concentration was determined to be 275 pg/mL in Jurkat cell culture supernatant. There was no detectable signal in THP-1 cell culture supernatant.
Interpolated concentrations of native CD28 in Jurkat and THP-1 extract samples based on a 1000 µg/ml extract load. Capture: HA723457, Human CD28 Rabbit mAb [PSH12-50] Detector: HA723458, Human CD28 Rabbit mAb [PSH12-51] Interpolated concentration of native CD28 was measured in duplicate at different sample concentrations and interpolated from the CD28 standard curves. The mean CD28 concentration was determined to be 4,345 pg/mL in Jurkat cell extract. There was no detectable signal in THP-1 cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-51)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human CD28 matched pair antibodies Capture: HA723457, Human CD28 Rabbit mAb [PSH12-50] Detector: HA723458, Human CD28 Rabbit mAb [PSH12-51] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723457) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD28 protein (HA210920) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723458, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CD28 in human samples. Capture: HA723457, Human CD28 Rabbit mAb [PSH12-50] Detector: HA723458, Human CD28 Rabbit mAb [PSH12-51] Interpolated concentration of native CD28 was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The mean CD28 concentration was determined to be 275 pg/mL in Jurkat cell culture supernatant. There was no detectable signal in THP-1 cell culture supernatant.
Interpolated concentrations of native CD28 in Jurkat and THP-1 extract samples based on a 1000 µg/ml extract load. Capture: HA723457, Human CD28 Rabbit mAb [PSH12-50] Detector: HA723458, Human CD28 Rabbit mAb [PSH12-51] Interpolated concentration of native CD28 was measured in duplicate at different sample concentrations and interpolated from the CD28 standard curves. The mean CD28 concentration was determined to be 4,345 pg/mL in Jurkat cell extract. There was no detectable signal in THP-1 cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-51)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human CD28 matched pair antibodies Capture: HA723457, Human CD28 Rabbit mAb [PSH12-50] Detector: HA723458, Human CD28 Rabbit mAb [PSH12-51] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723457) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD28 protein (HA210920) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723458, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CD28 in human samples. Capture: HA723457, Human CD28 Rabbit mAb [PSH12-50] Detector: HA723458, Human CD28 Rabbit mAb [PSH12-51] Interpolated concentration of native CD28 was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The mean CD28 concentration was determined to be 275 pg/mL in Jurkat cell culture supernatant. There was no detectable signal in THP-1 cell culture supernatant.
Interpolated concentrations of native CD28 in Jurkat and THP-1 extract samples based on a 1000 µg/ml extract load. Capture: HA723457, Human CD28 Rabbit mAb [PSH12-50] Detector: HA723458, Human CD28 Rabbit mAb [PSH12-51] Interpolated concentration of native CD28 was measured in duplicate at different sample concentrations and interpolated from the CD28 standard curves. The mean CD28 concentration was determined to be 4,345 pg/mL in Jurkat cell extract. There was no detectable signal in THP-1 cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier