CD19
HUABIO
catalog: EM40308

mouse monoclonal (A3-A8)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM40308, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Daudi cell lysate

Western blot analysis of CD19 on mouse lymphoid lysates with Mouse anti-CD19 antibody (EM40308) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40308) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40308, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

CD19
HUABIO
catalog: ET1702-74

domestic rabbit monoclonal (JF099-9)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of CD19 on different lysates with Rabbit anti-CD19 antibody (ET1702-74) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 µg/Lane) Lane 2: Ramos cell lysate (15 µg/Lane) Lane 3: PC-12 cell lysate (15 µg/Lane) Lane 4: Mouse spleen tissue lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 61 kDa Observed band size: 95 kDa Exposure time: 1 minute 14 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-74) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of PC-12 cells labeling CD19. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-74, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

CD19
HUABIO
catalog: ET1702-93-50UL

domestic rabbit monoclonal (JF100-06)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD19 on different lysates with Rabbit anti-CD19 antibody (ET1702-93) at 1/10,000 dilution. Lane 1: Raji cell lysate Lane 2: Ramos cell lysate Lane 3: 293T cell lysate (negative) Lane 4: Daudi cell lysate Lane 5: K-562 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 90 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-93) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Raji (positive) and 293T (negative) labeling CD19 with Rabbit anti-CD19 antibody (ET1702-93) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD19 antibody (ET1702-93) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD19 antibody (ET1702-93) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-93) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier

CD19
HUABIO
catalog: HA601518F

mouse monoclonal (PSH14-97)
reactivity: human
conjugate: FITC
application: flow cytometry

Flow cytometric analysis of human peripheral blood lymphocytes labeling CD19 (HA601518F, FITC) and CD3 (HA600118F, APC). Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with CD19 (HA601518F, FITC, 1/1,000) compared with Mouse IgG Isotype Control (FITC, 1/1,000).
quantity: 100T
price: 159.00 USD
to the supplier

CD19
HUABIO
catalog: HA723533

domestic rabbit monoclonal (PSH13-44)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD19 on different lysates with Rabbit anti-CD19 antibody (HA723533) at 1/50,000 dilution. Lane 1: Raji cell lysate Lane 2: Ramos cell lysate Lane 3: 293T cell lysate (negative) Lane 4: Daudi cell lysate Lane 5: K-562 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 100 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723533) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Raji (positive) and 293T (negative) labeling CD19 with Rabbit anti-CD19 antibody (HA723533) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD19 antibody (HA723533) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of K-562 (left, negative) and Ramos (right, positive) cells labeling CD19. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723533, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

CD19
HUABIO
catalog: HA723761F

domestic rabbit monoclonal (PSH13-44)
reactivity: human
conjugate: APC
application: flow cytometry

Flow cytometric analysis of human peripheral blood cells labelling CD19 (HA723761F, APC) and CD3 (HA600116F, FITC). Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with CD19 (HA723761F, APC, 1/1,000) compared with Rabbit IgG Isotype Control (APC, 1/1,000).

Flow cytometric analysis of K-562 (left, negative) and Ramos (right, positive) cells labeling CD19 (HA723761F, APC). Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with CD19 (HA723761F, APC, 1/1,000) (red). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100T
price: 189.00 USD
to the supplier

CD19
HUABIO
catalog: HA723762F

domestic rabbit monoclonal (PSH13-44)
reactivity: human
conjugate: FITC
application: flow cytometry

Flow cytometric analysis of human peripheral blood cells labelling CD19 (HA723762F, FITC) and CD3 (HA600118F, APC). Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with CD19 (HA723762F, FITC, 1/1,000) compared with Rabbit IgG Isotype Control (FITC, 1/1,000).
quantity: 100T
price: 149.00 USD
to the supplier

CD19
HUABIO
catalog: HA723763F

domestic rabbit monoclonal (PSH13-44)
reactivity: human
conjugate: PE
application: flow cytometry

Flow cytometric analysis of human peripheral blood cells labelling CD19 (HA723763F, PE) and CD3 (HA600116F, FITC). Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with CD19 (HA723763F, PE, 1/1,000) compared with Rabbit IgG Isotype Control (PE, 1/1,000).
quantity: 100T
price: 169.00 USD
to the supplier

CD19
HUABIO
catalog: HA750365

domestic rabbit monoclonal (JF100-06)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD19 on different lysates with Rabbit anti-CD19 antibody (HA750365) at 1/10,000 dilution. Lane 1: Raji cell lysate Lane 2: Ramos cell lysate Lane 3: 293T cell lysate (negative) Lane 4: Daudi cell lysate Lane 5: K-562 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 90 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750365) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Raji (positive) and 293T (negative) labeling CD19 with Rabbit anti-CD19 antibody (HA750365) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD19 antibody (HA750365) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD19 antibody (HA750365) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750365) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

CD19
HUABIO
catalog: HA751463

domestic rabbit monoclonal (PSH13-44)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD19 on different lysates with Rabbit anti-CD19 antibody (HA751463) at 1/50,000 dilution. Lane 1: Raji cell lysate Lane 2: Ramos cell lysate Lane 3: 293T cell lysate (negative) Lane 4: Daudi cell lysate Lane 5: K-562 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 100 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751463) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Raji (positive) and 293T (negative) labeling CD19 with Rabbit anti-CD19 antibody (HA751463) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD19 antibody (HA751463) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of K-562 (left, negative) and Ramos (right, positive) cells labeling CD19. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751463, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier

CD19
HUABIO
catalog: IRS018

domestic rabbit monoclonal
reactivity: human

mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD19 antibody (IRS018) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD19 antibody (IRS018) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 385.00 USD
to the supplier

CD19
HUABIO
catalog: R1107-7

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot analysis on F9 (A) and NCCIT (B) cell lysates using anti-CD19 rabbit polyclonal antibody.
quantity: 100μl
price: 330 USD
to the supplier