mouse monoclonal (A5B3)
reactivity: Escherichia virus M13
conjugate: HRP
application: ELISA
reactivity: Escherichia virus M13
conjugate: HRP
application: ELISA

Elisa Test of M13 antibody (HRP) Binding to M13 Phage. Samples were blocked with 1% BSA for 1 hour at 37℃ and incubated with the primary antibody (EM1902-18, 1/10000) for 1 hour at 25℃. The M13 antibody was repeatedly frozen and thawed for 4 times at -80℃, 4℃, 37℃.
quantity: 100μl
price: 384.00 USD
to the supplier
mouse monoclonal (A4C11)
reactivity: Escherichia virus M13
conjugate: HRP
application: ELISA
reactivity: Escherichia virus M13
conjugate: HRP
application: ELISA

Elisa Test of M13 antibody (HRP) Binding to M13 Phage. Samples were blocked with 1% BSA for 1 hour at 37℃ and incubated with the primary antibody (EM1902-19, 1/10000) for 1 hour at 25℃. The M13 antibody was repeatedly frozen and thawed for 4 times at -80℃, 4℃, 37℃.
quantity: 100μl
price: 384.00 USD
to the supplier
mouse monoclonal (PS00-01)
reactivity: Escherichia virus M13
application: ELISA
reactivity: Escherichia virus M13
application: ELISA

Indirect ELISA analysis of M13 was performed by coating HSA/BSA (2 μg/mL in pH 9.6 carbonate buffer) in 96-well plate, and then add the indicated amounts of M13 Bacteriophages displaying anti-HSA Fab overnight at 4℃. Incubated with 100 µl per well of anti-M13 monoclonal antibody starting at a dilution of 1/2,500 and serially diluting it to a dilution of 1:160,000 for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Mouse IgG secondary antibody (HA1006) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 360.00 USD
to the supplier
mouse monoclonal (PS00-01)
reactivity: Escherichia virus M13
conjugate: HRP
application: ELISA
reactivity: Escherichia virus M13
conjugate: HRP
application: ELISA
