CD8 alpha
HUABIO
catalog: ET1606-31

domestic rabbit monoclonal (SI18-01)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD8 alpha on different lysates with Rabbit anti-CD8 alpha antibody (ET1606-31) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: PC-3M cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-31) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-31, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-CD8 alpha antibody (ET1606-31) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-31) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Human CD8 alpha
HUABIO
catalog: HA600122F

mouse monoclonal (PSH04-94)
reactivity: human
conjugate: FITC
application: flow cytometry

Flow cytometric analysis of human peripheral blood lymphocytes labelling Human CD8 alpha (HA600122F, FITC) and CD3 (HA600118F, APC).

Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD8 alpha. Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with Human CD8 alpha (HA600122F, red, 2μg/mL). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 394.00 USD
to the supplier

Human CD8 alpha
HUABIO
catalog: HA600123F

mouse monoclonal (PSH04-94)
reactivity: human
conjugate: PE
application: flow cytometry

Flow cytometric analysis of human peripheral blood lymphocytes labelling Human CD8 alpha (HA600123F, PE) and CD3 (HA600116F, FITC).
quantity: 100μl
price: 360.00 USD
to the supplier

Human CD8 alpha
HUABIO
catalog: HA600124F

mouse monoclonal (PSH04-94)
reactivity: human
conjugate: APC
application: flow cytometry

Flow cytometric analysis of human peripheral blood lymphocytes labelling Human CD8 alpha (HA600124F, APC) and CD3 (HA600116F, FITC).

Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD8 alpha. Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with Human CD8 alpha (HA600124F, red, 2μg/mL). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 360.00 USD
to the supplier

Human CD8 alpha
HUABIO
catalog: HA601339

mouse monoclonal (PSH04-94)
reactivity: human
application: flow cytometry

Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD8 alpha. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601339, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; blue).
quantity: 100μl
price: 360.00 USD
to the supplier

CD8 alpha
HUABIO
catalog: HA750101

domestic rabbit monoclonal (SI18-01)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD8 alpha on different lysates with Rabbit anti-CD8 alpha antibody (HA750101) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: PC-3M cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750101) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750101, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-CD8 alpha antibody (HA750101) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-31) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier