domestic rabbit monoclonal (JE25-41)
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CD5 on human skin tissue lysates lysates with Rabbit anti-CD5 antibody (ET7110-56) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 50 kDa Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-56) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue tissue with Rabbit anti-CD5 antibody (ET7110-56) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-56) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD5 antibody (ET7110-56) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-56) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-01)
reactivity: human
conjugate: FITC
application: flow cytometry
reactivity: human
conjugate: FITC
application: flow cytometry
Flow cytometric analysis of human peripheral blood labelling Human CD5 (HA720211F, FITC).
quantity: 100μl
price: 429.00 USD
to the supplier
domestic rabbit monoclonal (PD00-01)
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD5 antibody (HA721137) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721137) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD5 antibody (HA721137) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721137) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application: IF-Tissue Species: Human Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/200
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-01)
reactivity: human
application: flow cytometry
reactivity: human
application: flow cytometry
Immunocytochemistry analysis of Jurkat (positive) and 293T (negative) labeling Human CD5 with Rabbit anti-Human CD5 antibody (HA722210) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Human CD5 antibody (HA722210) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD5. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722210, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier