CD3 epsilon
HUABIO
catalog: ER80501
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of CD3 epsilon on different lysates with Rabbit anti-CD3 epsilon antibody (ER80501) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (40 µg/Lane) Lane 3: Mouse thymus tissue lysate (40 µg/Lane) Lane 4: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER80501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
CD3 epsilon
HUABIO
catalog: ET1607-29
domestic rabbit monoclonal (SY0239)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD3 epsilon on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human thymus tissue lysate Lane 2: Jurkat cell lysate

Application: IF-Tissue Species: Human Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/1,000

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD3 epsilon antibody (ET1607-29) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
CD3E
HUABIO
catalog: HA500344
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of CD3E on different lysates with Rabbit anti-CD3E antibody (HA500344) at 1/500 dilution. Lane 1: Jurkat cell lysate (10 µg/Lane) Lane 2: Mouse thymus tissue lysate Lane 3: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500344) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
Human CD3
HUABIO
catalog: HA600116F
mouse monoclonal (PSH04-92)
reactivity: human
conjugate: FITC
application: flow cytometry

Flow cytometric analysis of human peripheral blood lymphocytes labelling Human CD3 (HA600116F, FITC).
quantity: 100μl
price: 394.00 USD
to the supplier
Human CD3
HUABIO
catalog: HA600117F
mouse monoclonal (PSH04-92)
reactivity: human
conjugate: PE
application: flow cytometry

Flow cytometric analysis of human peripheral blood lymphocytes labelling Human CD3 (HA600117F, PE).
quantity: 100μl
price: 360.00 USD
to the supplier
Human CD3
HUABIO
catalog: HA600118F
mouse monoclonal (PSH04-92)
reactivity: human
conjugate: APC
application: flow cytometry

Flow cytometric analysis of human peripheral blood lymphocytes labelling Human CD3 (HA600118F, APC).
quantity: 100μl
price: 360.00 USD
to the supplier
CD3
HUABIO
catalog: HA601110
mouse monoclonal (PD01-43)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (HA601110) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601110) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD3 antibody (HA601110) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601110) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD3 antibody (HA601110) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601110) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
Human CD3
HUABIO
catalog: HA601337
mouse monoclonal (PSH04-92)
reactivity: human
application: flow cytometry

Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD3. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601337, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 360.00 USD
to the supplier
CD3
HUABIO
catalog: HA720082
domestic rabbit monoclonal (JE80-02)
reactivity: human
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of CD3 on Jurkat cell lysates with Rabbit anti-CD3 antibody (HA720082) at 0.5μg/ml dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720082) at 0.5μg/ml dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Fluorescence multiplex immunohistochemical analysis of Tertiary Lymphoid Structures in Human Small Cell Lung Cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD20 (HA721138, green), anti-PD-L1 (HA721176, cyan), anti-CD56 (ET1702-43, magenta) and anti-CD3 (HA720082, yellow) on tertiary lymphoid structures. Panel B: anti- CD20 stained on B cells. Panel C: anti-PD-L1 stained on dendritic cells and macrophages cells. Panel D: anti-CD56 stained on NKT cells. Panel E: anti-CD3 stained on T cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in four rounds of staining: in the order of HA721138 (1/1,500 dilution), HA721176 (1/1,000 dilution), ET1702-43 (1/1,000 dilution), and HA720082 (1/500 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Fluorescence multiplex immunohistochemical analysis of the human non-small cell lung cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD20 (HA721138, green), anti-CD68 (HA601115, gray), anti-PD-L1 (HA721176, cyan), anti-panCK (HA601138, magenta) and anti-CD3 (HA720082, yellow) on human non-small cell lung cancer. Panel B: anti- CD20 stained on B cells. Panel C: anti-CD68 stained on macrophage M1 and macrophage M2. Panel D: anti-PD-L1 stained on dendritic cells and macrophages cells. Panel E: anti-panCK stained on cancer cells. Panel F: anti-CD3 stained on T cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in five rounds of staining: in the order of HA721138 (1/1,500 dilution), HA601115 (1/2,000 dilution), HA721176 (1/1,000 dilution), HA601138 (1/3,000 dilution), and HA720082 (1/500 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 385.00 USD
to the supplier
CD3 epsilon
HUABIO
catalog: HA750115
domestic rabbit monoclonal (SY0239)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD3 epsilon on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750115, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human thymus tissue lysate Lane 2: Jurkat cell lysate

Application: IF-Tissue Species: Human Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/1,000

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD3 epsilon antibody (HA750115) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750115) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
CD3
HUABIO
catalog: IRS022
domestic rabbit monoclonal
reactivity: human

mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with panCK (IRS010), CD3 (IRS022) and Ki67 (IRS036) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11b (IRS014), CD31 (IRS023), aSMA (IRS012), panCK (IRS010) and CD3 (IRS022) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD3 antibody (IRS022) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 385.00 USD
to the supplier