CD133
HUABIO
catalog: 0804-5

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of CD133 on HT-29 cell lysates with Rabbit anti-CD133 antibody (0804-5) at 1/500 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minute 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0804-5) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD133 on human stomach tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD133 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

CD133
HUABIO
catalog: ER1901-63

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (ER1901-63) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (10 µg/Lane) Lane 2: HT-29 cell lysate (10 µg/Lane) Lane 3: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-63) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

CD133
HUABIO
catalog: ER31008

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD133 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER31008, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SW480 cell lysate Lane 3: HCT116 cell lysate Lane 4: HT-29 cell lysate Lane 5: HepG2 cell lysate Lane 6: 293 cell lysate

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD133 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31008, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of SHG-44 cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
quantity: 100μl
price: 330 USD
to the supplier

CD133
HUABIO
catalog: HA601024

mouse monoclonal (A8C3)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA601024) at 1/2,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: HT-29 cell lysate Lane 3: NCCIT cell lysate Lane 4: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA601024) at 1/2,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 120/97 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA601024) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (10 µg/Lane) Lane 2: HT-29 cell lysate (10 µg/Lane) Lane 3: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601024) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

CD133
HUABIO
catalog: HA601025

mouse monoclonal (A8C2)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA601025) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (10 µg/Lane) Lane 2: HT-29 cell lysate (10 µg/Lane) Lane 3: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601025) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (HA601025) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601025) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-CD133 antibody (HA601025) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601025) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

CD133
HUABIO
catalog: HA601062

mouse monoclonal (A8C3-R)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA601062) at 1/1,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate (negative) Lane 4: NCCIT cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601062) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA601062) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601062) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (HA601062) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601062) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

CD133
HUABIO
catalog: HA610009

mouse monoclonal (A8C3-R)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA610009) at 1/1,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate (negative) Lane 4: NCCIT cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610009) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA610009) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610009) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (HA610009) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610009) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier