Neuropilin 1
HUABIO
catalog: ET1609-69-50UL

domestic rabbit monoclonal (ST05-30)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Neuropilin 1 on different lysates with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: A549 cell lysate Lane 3: SK-Br-3 cell lysate (low expression) Lane 4: MDA-MB-231 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 130 kDa Exposure time: 35 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-69) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western blot analysis of Neuropilin 1 on different lysates with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: SK-Br-3 cell lysate (low expression) Lane 4: Mouse brain tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 130 kDa Exposure time: Lane 1-3: 1 minute 21 seconds; Lane 4-7: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-69) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
quantity: 50μl
price: 205.00 USD
to the supplier

Human Neuropilin-1
HUABIO
catalog: HA725112

domestic rabbit monoclonal (PSH14-73)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Neuropilin-1 matched pair antibodies Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73] Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725112) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropilin-1 protein (HA211017) starting from 125,000 pg/ml to 0 pg/ml and detect antibody (HA725113, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Neuropilin-1 in HT-29 and SK-BR-3 extract samples based on a 1,000 µg/ml extract load. Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73] Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74] The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curve and corrected for sample dilution. Undiluted samples are HT-29 extract 50% and SK-BR-3 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 7,020 pg/ml in HT-29 extract and undetectable in SK-BR-3 extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Neuropilin-1
HUABIO
catalog: HA725113

domestic rabbit monoclonal (PSH14-74)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Neuropilin-1 matched pair antibodies Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73] Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725112) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropilin-1 protein (HA211017) starting from 125,000 pg/ml to 0 pg/ml and detect antibody (HA725113, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Neuropilin-1 in HT-29 and SK-BR-3 extract samples based on a 1,000 µg/ml extract load. Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73] Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74] The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curve and corrected for sample dilution. Undiluted samples are HT-29 extract 50% and SK-BR-3 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 7,020 pg/ml in HT-29 extract and undetectable in SK-BR-3 extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Neuropilin-1
HUABIO
catalog: HA725114H

domestic rabbit monoclonal (PSH14-74)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human Neuropilin-1 matched pair antibodies Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73] Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725112) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropilin-1 protein (HA211017) starting from 125,000 pg/ml to 0 pg/ml and detect antibody (HA725113, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Neuropilin-1 in HT-29 and SK-BR-3 extract samples based on a 1,000 µg/ml extract load. Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73] Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74] The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curve and corrected for sample dilution. Undiluted samples are HT-29 extract 50% and SK-BR-3 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 7,020 pg/ml in HT-29 extract and undetectable in SK-BR-3 extract.
quantity: 100μl
price: 409.00 USD
to the supplier

Neuropilin 1
HUABIO
catalog: HA750189

domestic rabbit monoclonal (ST05-30)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Neuropilin 1 on different lysates with Rabbit anti-Neuropilin 1 antibody (HA750189) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: A549 cell lysate Lane 3: SK-Br-3 cell lysate (low expression) Lane 4: MDA-MB-231 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 130 kDa Exposure time: 35 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750189) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Neuropilin 1 antibody (HA750189) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750189) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western blot analysis of Neuropilin 1 on different lysates with Rabbit anti-Neuropilin 1 antibody (HA750189) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: SK-Br-3 cell lysate (low expression) Lane 4: Mouse brain tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 130 kDa Exposure time: Lane 1-3: 1 minute 21 seconds; Lane 4-7: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750189) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Neuropilin 1
HUABIO
catalog: M0910-5

mouse monoclonal (A3-D1)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis on recombinant protein using anti-Neuropilin 1 Mouse mAb (Cat. # M0910-5).

Immunofluorescent staining of Hela cells using anti-Neuropilin 1 Mouse mAb (Cat. # M0910-5).

Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-Neuropilin 1 Mouse mAb (Cat. # M0910-5).
quantity: 100μl
price: 360.00 USD
to the supplier