TNFRSF14
HUABIO
catalog: HA722303
domestic rabbit monoclonal (PSH05-56)
reactivity: human
application: western blot

Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody (HA722303) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: SK-MEL-28 cell lysate treated with deglycosylation Lane 3: A549 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 40-50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722303) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
TNFRSF14
HUABIO
catalog: HA722501
domestic rabbit monoclonal (PSH06-02)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody (HA722501) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: SK-MEL-28 cell lysate treated with deglycosylation Lane 3: A549 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 40-50 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SK-MEL-28 (positive) and A549 (negative) labeling TNFRSF14 with Rabbit anti-TNFRSF14 antibody (HA722501) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TNFRSF14 antibody (HA722501) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of SK-MEL-28 (left, positive) and A549 (right, negative) cells labeling TNFRSF14. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722501, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
TNFRSF14/HVEM
HUABIO
catalog: HA722676
domestic rabbit monoclonal (PSH06-41)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human HVEM matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722676) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human TNFRSF14 protein (HA210556) starting from 4,500 pg/ml to 0 pg/ml and detect antibody (HA722677, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native HVEM in human serum samples. The concentrations of HVEM were measured in triplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean HVEM concentration was determined to be 5,867 pg/ml in human serum.

Interpolated concentrations of native HVEM in Raji and HL-60 extract samples based on a 1,000 µg/ml extract load. The concentrations of HVEM were measured in duplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are Raji extract 13% and HL-60 extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HVEM concentration was determined to be 3,332 pg/ml in Raji extract and 1,901 pg/ml in HL-60 extract.
quantity: 100μl
price: 649.00 USD
to the supplier
TNFRSF14/HVEM
HUABIO
catalog: HA722677
domestic rabbit monoclonal (PSH06-42)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human HVEM matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722676) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human TNFRSF14 protein (HA210556) starting from 4,500 pg/ml to 0 pg/ml and detect antibody (HA722677, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native HVEM in human serum samples. The concentrations of HVEM were measured in triplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean HVEM concentration was determined to be 5,867 pg/ml in human serum.

Interpolated concentrations of native HVEM in Raji and HL-60 extract samples based on a 1,000 µg/ml extract load. The concentrations of HVEM were measured in duplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are Raji extract 13% and HL-60 extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HVEM concentration was determined to be 3,332 pg/ml in Raji extract and 1,901 pg/ml in HL-60 extract.
quantity: 100μl
price: 649.00 USD
to the supplier
TNFRSF14/HVEM
HUABIO
catalog: HA722678B
domestic rabbit monoclonal (PSH06-42)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human HVEM matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722676) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human TNFRSF14 protein (HA210556) starting from 4,500 pg/ml to 0 pg/ml and detect antibody (HA722678B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native HVEM in human serum samples. The concentrations of HVEM were measured in triplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean HVEM concentration was determined to be 5,867 pg/ml in human serum.

Interpolated concentrations of native HVEM in Raji and HL-60 extract samples based on a 1,000 µg/ml extract load. The concentrations of HVEM were measured in duplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are Raji extract 13% and HL-60 extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HVEM concentration was determined to be 3,332 pg/ml in Raji extract and 1,901 pg/ml in HL-60 extract.
quantity: 100μl
price: 409.00 USD
to the supplier
TNFRSF14
HUABIO
catalog: HA751018
domestic rabbit monoclonal (PSH05-56)
reactivity: human
application: western blot

Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody (HA751018) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: SK-MEL-28 cell lysate treated with deglycosylation Lane 3: A549 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 40-50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751018) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
TNFRSF14
HUABIO
catalog: HA751042
domestic rabbit monoclonal (PSH06-02)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody (HA751042) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: SK-MEL-28 cell lysate treated with deglycosylation Lane 3: A549 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 40-50 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751042) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SK-MEL-28 (positive) and A549 (negative) labeling TNFRSF14 with Rabbit anti-TNFRSF14 antibody (HA751042) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TNFRSF14 antibody (HA751042) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of SK-MEL-28 (left, positive) and A549 (right, negative) cells labeling TNFRSF14. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751042, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier