domestic rabbit monoclonal (PSH08-05)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human TRAIL matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722943) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human TRAIL protein as the standard (HA210893) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722944, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native TRAIL in 786-0 and Hela extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TRAIL concentration was determined to be 11,821 pg/mL in 786-0 cell extract, There was no detectable signal in Hela cell extract.
Interpolated concentrations of native TRAIL in cell culture supernatant samples. Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean concentration was determined to be 260 pg/mL in 786-0 cell supernanant. There was no detectable signal in Hela cell supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-06)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human TRAIL matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722943) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human TRAIL protein as the standard (HA210893) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722944, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native TRAIL in 786-0 and Hela extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TRAIL concentration was determined to be 11,821 pg/mL in 786-0 cell extract, There was no detectable signal in Hela cell extract.
Interpolated concentrations of native TRAIL in cell culture supernatant samples. Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean concentration was determined to be 260 pg/mL in 786-0 cell supernanant. There was no detectable signal in Hela cell supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-06)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human TRAIL matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722943) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human TRAIL protein as the standard (HA210893) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722944, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native TRAIL in 786-0 and Hela extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TRAIL concentration was determined to be 11,821 pg/mL in 786-0 cell extract, There was no detectable signal in Hela cell extract.
Interpolated concentrations of native TRAIL in cell culture supernatant samples. Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean concentration was determined to be 260 pg/mL in 786-0 cell supernanant. There was no detectable signal in Hela cell supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-40)
reactivity: human
application: western blot, flow cytometry
reactivity: human
application: western blot, flow cytometry
Western blot analysis of TRAIL on different lysates with Rabbit anti-TRAIL antibody (HA723634) at 1/5,000 dilution. Lane 1: 786-0 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 28/30 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723634) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HeLa (left, negative) and 786-0 (right, positive) cells labeling TRAIL. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723634, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-40)
reactivity: human
application: western blot, flow cytometry
reactivity: human
application: western blot, flow cytometry
Western blot analysis of TRAIL on different lysates with Rabbit anti-TRAIL antibody (HA751522) at 1/5,000 dilution. Lane 1: 786-0 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 28/30 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751522) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HeLa (left, negative) and 786-0 (right, positive) cells labeling TRAIL. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751522, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier