DR3 / LARD
HUABIO
catalog: HA723583
domestic rabbit monoclonal (PSH13-90)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of DR3 / LARD on different lysates with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/5,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: THP-1 cell lysate (20 µg/Lane) Lane 4: HDLM-2 cell lysate (20 µg/Lane) Lane 5: U-87 MG cell lysate (20 µg/Lane) Lane 6: HeLa cell lysate (20 µg/Lane) Lane 7: Neuro-2a cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Lane 11: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723583) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Jurkat cells labeling DR3 / LARD with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
Human DR3/TNFRSF25
HUABIO
catalog: HA723869
domestic rabbit monoclonal (PSH16-67)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human DR3/TNFRSF25 matched pair antibodies Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723869) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DR3/TNFRSF25 protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723870, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human DR3/TNFRSF25 matched pair antibodies Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723869) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DR3/TNFRSF25 protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723871, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native DR3/TNFRSF25 in TF-1 and HUVEC extract samples based on a 1,000 µg/ml extract load. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] The concentrations of DR3/TNFRSF25 were measured in duplicates, interpolated from the DR3/TNFRSF25 standard curve and corrected for sample dilution. Undiluted samples are TF-1 extract 25% and HUVEC extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DR3/TNFRSF25 concentration was determined to be 1,188 pg/ml in TF-1 extract and undetectable in HUVEC extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human DR3/TNFRSF25
HUABIO
catalog: HA723870
domestic rabbit monoclonal (PSH16-68)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human DR3/TNFRSF25 matched pair antibodies Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723869) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DR3/TNFRSF25 protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723870, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native DR3/TNFRSF25 in TF-1 and HUVEC extract samples based on a 1,000 µg/ml extract load. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] The concentrations of DR3/TNFRSF25 were measured in duplicates, interpolated from the DR3/TNFRSF25 standard curve and corrected for sample dilution. Undiluted samples are TF-1 extract 25% and HUVEC extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DR3/TNFRSF25 concentration was determined to be 1,188 pg/ml in TF-1 extract and undetectable in HUVEC extract.

Interpolated concentrations of spiked DR3/TNFRSF25 in human cell culture media samples. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] The concentrations of DR3/TNFRSF25 were measured in triplicates, interpolated from the DR3/TNFRSF25 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).
quantity: 100μl
price: 649.00 USD
to the supplier
Human DR3/TNFRSF25
HUABIO
catalog: HA723870B
domestic rabbit monoclonal (PSH16-68)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human DR3/TNFRSF25 matched pair antibodies Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723869) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DR3/TNFRSF25 protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723870, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native DR3/TNFRSF25 in TF-1 and HUVEC extract samples based on a 1,000 µg/ml extract load. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] The concentrations of DR3/TNFRSF25 were measured in duplicates, interpolated from the DR3/TNFRSF25 standard curve and corrected for sample dilution. Undiluted samples are TF-1 extract 25% and HUVEC extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DR3/TNFRSF25 concentration was determined to be 1,188 pg/ml in TF-1 extract and undetectable in HUVEC extract.

Interpolated concentrations of spiked DR3/TNFRSF25 in human cell culture media samples. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] The concentrations of DR3/TNFRSF25 were measured in triplicates, interpolated from the DR3/TNFRSF25 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).
quantity: 100μl
price: 409.00 USD
to the supplier
Human DR3/TNFRSF25
HUABIO
catalog: HA723871
domestic rabbit monoclonal (PSH16-69)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human DR3/TNFRSF25 matched pair antibodies Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723869) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DR3/TNFRSF25 protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723871, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native DR3/TNFRSF25 in TF-1 and HUVEC extract samples based on a 1,000 µg/ml extract load. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] The concentrations of DR3/TNFRSF25 were measured in duplicates, interpolated from the DR3/TNFRSF25 standard curve and corrected for sample dilution. Undiluted samples are TF-1 extract 25% and HUVEC extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DR3/TNFRSF25 concentration was determined to be 2,154 pg/ml in TF-1 extract and undetectable in HUVEC extract.

Interpolated concentrations of spiked DR3/TNFRSF25 in human cell culture media samples. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] The concentrations of DR3/TNFRSF25 were measured in triplicates, interpolated from the DR3/TNFRSF25 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).
quantity: 100μl
price: 649.00 USD
to the supplier
Human DR3/TNFRSF25
HUABIO
catalog: HA723871B
domestic rabbit monoclonal (PSH16-69)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human DR3/TNFRSF25 matched pair antibodies Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723869) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DR3/TNFRSF25 protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723871, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native DR3/TNFRSF25 in TF-1 and HUVEC extract samples based on a 1,000 µg/ml extract load. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] The concentrations of DR3/TNFRSF25 were measured in duplicates, interpolated from the DR3/TNFRSF25 standard curve and corrected for sample dilution. Undiluted samples are TF-1 extract 25% and HUVEC extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DR3/TNFRSF25 concentration was determined to be 2,154 pg/ml in TF-1 extract and undetectable in HUVEC extract.

Interpolated concentrations of spiked DR3/TNFRSF25 in human cell culture media samples. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] The concentrations of DR3/TNFRSF25 were measured in triplicates, interpolated from the DR3/TNFRSF25 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).
quantity: 100μl
price: 409.00 USD
to the supplier
DR3 / LARD
HUABIO
catalog: HA751492
domestic rabbit monoclonal (PSH13-90)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of DR3 / LARD on different lysates with Rabbit anti-DR3 / LARD antibody (HA751492) at 1/5,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: THP-1 cell lysate (20 µg/Lane) Lane 4: HDLM-2 cell lysate (20 µg/Lane) Lane 5: U-87 MG cell lysate (20 µg/Lane) Lane 6: HeLa cell lysate (20 µg/Lane) Lane 7: Neuro-2a cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Lane 11: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751492) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Jurkat cells labeling DR3 / LARD with Rabbit anti-DR3 / LARD antibody (HA751492) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DR3 / LARD antibody (HA751492) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier