Human TSLP
HUABIO
catalog: HA723487

domestic rabbit monoclonal (PSH12-87)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human TSLP matched pair antibodies Capture: HA723487, Human TSLP Rabbit mAb [PSH12-87] Detector: HA723488, Human TSLP Rabbit mAb [PSH12-88] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723487) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (HA210952) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723488, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked TSLP in human cell culture media samples. The concentrations of TSLP were measured in duplicates, interpolated from the TSLPstandard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human TSLP
HUABIO
catalog: HA723488

domestic rabbit monoclonal (PSH12-88)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human TSLP matched pair antibodies Capture: HA723487, Human TSLP Rabbit mAb [PSH12-87] Detector: HA723488, Human TSLP Rabbit mAb [PSH12-88] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723487) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (HA210952) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723488, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked TSLP in human cell culture media samples. The concentrations of TSLP were measured in duplicates, interpolated from the TSLPstandard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human TSLP
HUABIO
catalog: HA723489B

domestic rabbit monoclonal (PSH12-88)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human TSLP matched pair antibodies Capture: HA723487, Human TSLP Rabbit mAb [PSH12-87] Detector: HA723488, Human TSLP Rabbit mAb [PSH12-88] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723487) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (HA210952) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723488, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked TSLP in human cell culture media samples. The concentrations of TSLP were measured in duplicates, interpolated from the TSLPstandard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

Human TSLP
HUABIO
catalog: HA723490

domestic rabbit monoclonal (PSH12-89)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human TSLP matched pair antibodies Capture: HA723490, Human TSLP Rabbit mAb [PSH12-89] Detector: HA723491, Human TSLP Rabbit mAb [PSH12-90] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723490) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (HA210952) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723491, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked TSLP in human cell culture media samples. The concentrations of TSLPwere interpolated from the TSLP standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 1 25%, cell culture media 2 50%.
quantity: 100μl
price: 649.00 USD
to the supplier

Human TSLP
HUABIO
catalog: HA723491

domestic rabbit monoclonal (PSH12-90)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human TSLP matched pair antibodies Capture: HA723490, Human TSLP Rabbit mAb [PSH12-89] Detector: HA723491, Human TSLP Rabbit mAb [PSH12-90] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723490) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (HA210952) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723491, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked TSLP in human cell culture media samples. The concentrations of TSLPwere interpolated from the TSLP standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 1 25%, cell culture media 2 50%.
quantity: 100μl
price: 649.00 USD
to the supplier

Human TSLP
HUABIO
catalog: HA723492B

domestic rabbit monoclonal (PSH12-90)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human TSLP matched pair antibodies Capture: HA723490, Human TSLP Rabbit mAb [PSH12-89] Detector: HA723491, Human TSLP Rabbit mAb [PSH12-90] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723490) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (HA210952) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723491, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked TSLP in human cell culture media samples. The concentrations of TSLPwere interpolated from the TSLP standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 1 25%, cell culture media 2 50%.
quantity: 100μl
price: 409.00 USD
to the supplier